Tested Applications
| Positive WB detected in | pig brain tissue, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human heart tissue, rat liver tissue, mouse liver tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68253-1-Ig targets NDUFS1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3135 Product name: Recombinant human NDUFS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-305 aa of BC030833 Sequence: MLRIPVRKALVGLSKSPKGCVRTTATAASNLIEVFVDGQSVMVEPGTTVLQACEKVGMQIPRFCYHERLSVAGNCRMCLVEIEKAPKVVAACAMPVMKGWNILTNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGNDRSRFLEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGNDMQVGTYIEKMFMSELSGNIIDICPVGALTSKPYAFTAQPWETRKTESIDVMDAVGSNIVVSTRTGEVMRILPRMHEDINEEWISDKTRFAYDGLKRQRLTEP Predict reactive species |
| Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) |
| Calculated Molecular Weight | 727 aa, 79 kDa |
| Observed Molecular Weight | 68-81 kDa |
| GenBank Accession Number | BC030833 |
| Gene Symbol | NDUFS1 |
| Gene ID (NCBI) | 4719 |
| RRID | AB_2935338 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P28331 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NDUFS1 antibody 68253-1-Ig | Download protocol |
| IHC protocol for NDUFS1 antibody 68253-1-Ig | Download protocol |
| WB protocol for NDUFS1 antibody 68253-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NDUFS1 Monoclonal antibody (3H5E8), catalog number 68253-1-Ig, has 4 total citations published in Sci China Life Sci, Acta Neuropathol Commun, Int J Med Sci, and Arch Oral Biol. The associated research fields span mitochondrial dysfunction in Parkinson's disease, aflatoxin B1 cytotoxicity via protein modifications, alveolar fluid clearance in acute lung injury, and disulfidptosis in periodontitis. Applications cited include WB, IF, and CoIP across human, rat, and pig species.
| Species | Application | Title |
|---|---|---|
Sci China Life Sci CRISPR screening identifies protein methylation and ubiquitination modifications that modulate aflatoxin B1 cytotoxicity | ||
Acta Neuropathol Commun BCKDK loss impairs mitochondrial Complex I activity and drives alpha-synuclein aggregation in models of Parkinson's disease | ||
Int J Med Sci NDUFS1 upregulates ENaCα by NAD+ to promote alveolar fluid clearance in acute lung injury | ||
Arch Oral Biol Correlation of disulfidptosis and periodontitis: New insights and clinical significance |
