NDUFS1 Monoclonal antibody
NDUFS1 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig, Rabbit
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3H5E8
Cat no : 68253-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig brain tissue, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68253-1-Ig targets NDUFS1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.
Tested Reactivity | Human, Mouse, Rat, Pig, Rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | NDUFS1 fusion protein Ag3135 |
Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) |
Calculated Molecular Weight | 727 aa, 79 kDa |
Observed Molecular Weight | 68-81 kDa |
GenBank Accession Number | BC030833 |
Gene Symbol | NDUFS1 |
Gene ID (NCBI) | 4719 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. NDUFS1 has some isoforms with the molecular mass of 68-81 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for NDUFS1 antibody 68253-1-Ig | Download protocol |
IHC protocol for NDUFS1 antibody 68253-1-Ig | Download protocol |
IF protocol for NDUFS1 antibody 68253-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |