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  • KD/KO Validated

NDUFS4 Polyclonal antibody

NDUFS4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15849-1-AP

Synonyms

AQDQ, CI 18 kDa, CI AQDQ, Complex I 18 kDa, Complex I AQDQ, NDUFS4



Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

15849-1-AP targets NDUFS4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFS4 fusion protein Ag8633
Full Name NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC005270
Gene symbol NDUFS4
Gene ID (NCBI) 4724
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFS4 antibody 15849-1-APDownload protocol
IHC protocol for NDUFS4 antibody 15849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Immunity

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Authors - Hongxu Xian
  • KO Validated
mouseWB

Theranostics

Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.

Authors - Lujing Jiang
  • KD Validated
mouseWB

EBioMedicine

SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice.

Authors - Yipeng Du
ratWB

Arch Biochem Biophys

Epigallocatechin-3-gallate exerts cardioprotective effects related to energy metabolism in pressure overload-induced cardiac dysfunction.

Authors - Qiuhong Mou