NDUFS4 Polyclonal antibody

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  WB analysis of mouse heart using 15849-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15849-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15849-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15849-1-AP (NDUFS4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15849-1-AP (NDUFS4 Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

NDUFS4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15849-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

AQDQ, CI 18 kDa, CI AQDQ, Complex I 18 kDa, Complex I AQDQ, NDUFS4

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

15849-1-AP targets NDUFS4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFS4 fusion protein Ag8633
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
• Calculated Molecular Weight: 175 aa, 20 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC005270
• Gene Symbol: NDUFS4
• Gene ID (NCBI): 4724
• RRID: AB_2878191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43181
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NDUFS4 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 mitochondrial), also known as AQDQ, is a member of NADH dehydrogenase (ubiquinone) iron-sulfur (NDUFS) protein family. It is a peripheral membrane protein located on the matrix side of the inner mitochondrial membrane. It is reported that the loss of NDUFS4 protein expression in immunohistochemistry (IHC) reliably diagnoses papillary thyroid carcinoma(PMID: 34792302). Also, NDUFS4 promotes tumor progression and predicts prognosis in gastric cancer(PMID: 36044738).

Publications

Species	Application	Title
mouse	WB	Immunity; Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.; Authors - Hongxu Xian ; KO Validated
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang
mouse	WB	EBioMedicine; SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice.; Authors - Yipeng Du
rat	WB	Arch Biochem Biophys; Epigallocatechin-3-gallate exerts cardioprotective effects related to energy metabolism in pressure overload-induced cardiac dysfunction.; Authors - Qiuhong Mou
		Cell Commun Signal; The dose-dependent dual effects of alpha-ketoglutarate (AKG) on cumulus oocyte complexes during in vitro maturation; Authors - Yunjie Liu
human	WB,IF	Sci Rep; Mitochondrial complex I inhibition enhances astrocyte responsiveness to pro-inflammatory stimuli; Authors - Lena Wischhof