NDUFS4 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDUFS4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15849-1-AP

Synonyms

AQDQ, CI 18 kDa, CI AQDQ, Complex I 18 kDa, Complex I AQDQ, NDUFS4

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Validation Data Gallery

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  WB analysis of mouse heart using 15849-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15849-1-AP (NDUFS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15849-1-AP (NDUFS4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15849-1-AP (NDUFS4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below

Product Information

15849-1-AP targets NDUFS4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFS4 fusion protein Ag8633
• Full Name: NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase)
• Calculated molecular weight: 175 aa, 20 kDa
• Observed molecular weight: 21 kDa
• GenBank accession number: BC005270
• Gene symbol: NDUFS4
• Gene ID (NCBI): 4724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Immunity; Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.; Authors - Hongxu Xian ; KO Validated
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang ; KD Validated
mouse	WB	EBioMedicine; SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice.; Authors - Yipeng Du
rat	WB	Arch Biochem Biophys; Epigallocatechin-3-gallate exerts cardioprotective effects related to energy metabolism in pressure overload-induced cardiac dysfunction.; Authors - Qiuhong Mou