Tested Applications
Positive WB detected in | A2780 cells, SKOV-3 cells, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
25172-1-AP targets NDUFS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen |
CatNo: Ag18531 Product name: Recombinant human NDUFS8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC119754 Sequence: MRCLTTPMLLRALAQAARAGPPGGRSLHSSAVAATYKYVNMQDPEMDMKSVTDRAARTLLWTELFRGLGMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR Predict reactive species |
Full Name | NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) |
Calculated Molecular Weight | 210 aa, 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC119754 |
Gene Symbol | NDUFS8 |
Gene ID (NCBI) | 4728 |
RRID | AB_2879939 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00217 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NADH: ubiquinone oxidoreductase core subunit S8 (NDUFS8), also called TYKY, is an essential core subunit and component of the iron-sulfur (FeS) fragment of mitochondrial complex I directly involved in the electron transfer process and energy metabolism (PMID: 36557887). Pathogenic variants of the NDUFS8 are relevant to infantile-onset and severe diseases, including Leigh syndrome, cancer, and diabetes mellitus (PMID: 36557887). Knockdown of NDUFS8 impaired mitochondrial function, increased ROS, disrupted redox homeostasis, induced apoptosis, and suppressed proliferation and migration of HCC cells (PMID: 40914145).
Protocols
Product Specific Protocols | |
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WB protocol for NDUFS8 antibody 25172-1-AP | Download protocol |
IHC protocol for NDUFS8 antibody 25172-1-AP | Download protocol |
IF protocol for NDUFS8 antibody 25172-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |