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NDUFS8 Recombinant monoclonal antibody, PBS Only (87156-1-PBS)

Filter: AllWBIHCIF/ICCAffinity

WB analysis using 87156-1-RR (same clone as 87156-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87156-1-RR (NDUFS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.
WB analysis using 87156-1-RR (same clone as 87156-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 87156-1-RR (NDUFS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 87156-1-RR (same clone as 87156-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87156-1-RR (NDUFS8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 87156-1-RR (same clone as 87156-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87156-1-RR (NDUFS8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 87156-1-RR (same clone as 87156-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFS8 antibody (87156-1-RR, Clone: 252267D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87156-1-PBS in a different storage buffer formulation.
Affinity of 87156-1-RR
Biolayer interferometry (BLl) kinetic assays of 87156-1-RR against Human NDUFS8 were performed. The affinity constant is below 1 pM.

NDUFS8 Recombinant monoclonal antibody, PBS Only

Name: NDUFS8 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 87156-1-PBS

NDUFS8 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 87156-1-PBS

Clone No.252267D5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CI 23kD, CI-23kD, Complex I 23kD, Complex I-23kD, EC:7.1.1.2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

87156-1-PBS targets NDUFS8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag18531 Product name: Recombinant human NDUFS8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC119754 Sequence: MRCLTTPMLLRALAQAARAGPPGGRSLHSSAVAATYKYVNMQDPEMDMKSVTDRAARTLLWTELFRGLGMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR Predict reactive species
Full Name	NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)
Calculated Molecular Weight	210 aa, 24 kDa
Observed Molecular Weight	24 kDa
GenBank Accession Number	BC119754
Gene Symbol	NDUFS8
Gene ID (NCBI)	4728
RRID	AB_3745377
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O00217
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NADH: ubiquinone oxidoreductase core subunit S8 (NDUFS8), also called TYKY, is an essential core subunit and component of the iron-sulfur (FeS) fragment of mitochondrial complex I directly involved in the electron transfer process and energy metabolism (PMID: 36557887). Pathogenic variants of the NDUFS8 are relevant to infantile-onset and severe diseases, including Leigh syndrome, cancer, and diabetes mellitus (PMID: 36557887). Knockdown of NDUFS8 impaired mitochondrial function, increased ROS, disrupted redox homeostasis, induced apoptosis, and suppressed proliferation and migration of HCC cells (PMID: 40914145).