NDUFV2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NDUFV2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

36

Cat no : 15301-1-AP

Synonyms

NDUFV2

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Validation Data Gallery

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  WB analysis using 15301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15301-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 15301-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15301-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 15301-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15301-1-AP (NDUFV2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15301-1-AP

  IP Result of anti-NDUFV2 (IP:15301-1-AP, 3ug; Detection:15301-1-AP 1:400) with mouse heart tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 15301-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15301-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15301-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15301-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15301-1-AP (NDUFV2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15301-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15301-1-AP (NDUFV2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with alpha tubulin antibody 66031-1-Ig and CoraLite®594-Conjugated AffiniPure Goat Anti-mouse IgG(H+L). Blue: DAPI.

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  IF Staining of HeLa using 15301-1-AP

  Immunofluorescent analysis of Hela cells, using NDUFV2 antibody 15301-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human prostate cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Product Information

15301-1-AP targets NDUFV2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NDUFV2 fusion protein Ag7559
• Full Name: NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
• Calculated molecular weight: 27 kDa
• Observed molecular weight: 24-27 kDa
• GenBank accession number: BC001632
• Gene symbol: NDUFV2
• Gene ID (NCBI): 4729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
human	WB	Mol Cell; Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.; Authors - Yilin Kang
human,mouse	WB,IP	Nat Commun; A salvage pathway maintains highly functional respiratory complex I.; Authors - Karolina Szczepanowska
mouse	WB	Dev Cell; Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.; Authors - Rani Ojha
mouse	WB	Nat Commun; Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice.; Authors - Satomi Miwa
mouse	WB	J Cell Biol; A keratin scaffold regulates epidermal barrier formation, mitochondrial lipid composition, and activity.; Authors - Vinod Kumar