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NDUFV2 Polyclonal antibody

NDUFV2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15301-1-AP



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman prostate cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15301-1-AP targets NDUFV2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFV2 fusion protein Ag7559
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 24-27 kDa
GenBank Accession NumberBC001632
Gene Symbol NDUFV2
Gene ID (NCBI) 4729
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).


Product Specific Protocols
WB protocol for NDUFV2 antibody 15301-1-APDownload protocol
IHC protocol for NDUFV2 antibody 15301-1-APDownload protocol
IF protocol for NDUFV2 antibody 15301-1-APDownload protocol
IP protocol for NDUFV2 antibody 15301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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