• Featured Product
  • KD/KO Validated

NDUFV2 Polyclonal antibody

NDUFV2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15301-1-AP

Synonyms

NDUFV2



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman prostate cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15301-1-AP targets NDUFV2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFV2 fusion protein Ag7559
Full Name NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 24-27 kDa
GenBank Accession NumberBC001632
Gene Symbol NDUFV2
Gene ID (NCBI) 4729
RRIDAB_2149048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).

Protocols

Product Specific Protocols
WB protocol for NDUFV2 antibody 15301-1-APDownload protocol
IHC protocol for NDUFV2 antibody 15301-1-APDownload protocol
IF protocol for NDUFV2 antibody 15301-1-APDownload protocol
IP protocol for NDUFV2 antibody 15301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
humanWB

Mol Cell

Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.

Authors - Yilin Kang
human,mouseWB,IP

Nat Commun

A salvage pathway maintains highly functional respiratory complex I.

Authors - Karolina Szczepanowska
mouseWB

Dev Cell

Regulation of reverse electron transfer at mitochondrial complex I by unconventional Notch action in cancer stem cells.

Authors - Rani Ojha
mouseWB

Nat Commun

Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice.

Authors - Satomi Miwa
mouseWB

J Cell Biol

A keratin scaffold regulates epidermal barrier formation, mitochondrial lipid composition, and activity.

Authors - Vinod Kumar