Validation Data Gallery
|Positive WB detected in||mouse heart tissue, rat heart tissue, rat skeletal muscle tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human prostate cancer tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15301-1-AP targets NDUFV2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NDUFV2 fusion protein Ag7559|
|Full Name||NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||24-27 kDa|
|GenBank accession number||BC001632|
|Gene ID (NCBI)||4729|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The NDUFV2 gene encodes the 24-kD subunit of the mitochondrial NADH:ubiquinone oxidoreductase (complex I of the respiratory chain). The protein belongs to the complex I 24 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. NDUFV2 constitutes one genetic risk factor for PD, and the mutation may well be a cause of complex I deficiency in this disease(PMID:9570948).
|Product Specific Protocols|
|WB protocol for NDUFV2 antibody 15301-1-AP||Download protocol|
|IHC protocol for NDUFV2 antibody 15301-1-AP||Download protocol|
|IF protocol for NDUFV2 antibody 15301-1-AP||Download protocol|
|IP protocol for NDUFV2 antibody 15301-1-AP||Download protocol|
|Click here to view our Standard Protocols|
BMC Res Notes
Subunit composition of respiratory chain complex 1 and its responses to oxygen in mitochondria from human donor livers.
CLPP deficiency protects against metabolic syndrome but hinders adaptive thermogenesis.
Cell Death Dis
Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca2+]m-PDP1-PDH-histone acetylation retrograde signaling.
Sex-specific genetic regulation of adipose mitochondria and metabolic syndrome by Ndufv2.
Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice.
J Genet Eng Biotechnol
The NDUFV2 gene silencing inhibits the proliferation of two drug-resistant cancer cell lines.