NECAB2 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• NECAB2 Recombinant monoclonal antibody (88290-1-RR)

NECAB2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88290-1-RR

Clone No.260727H8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EFCBP2, EF-hand calcium-binding protein 2, Neuronal calcium-binding protein 2, N-terminal EF-hand calcium-binding protein 2, Stip 2

• 

  WB analysis using 88290-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88290-1-RR (NECAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88290-1-RR targets NECAB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2909

  Product name: Recombinant human NECAB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 125-386 aa of BC016979

  Sequence: DHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD

• Full Name: N-terminal EF-hand calcium binding protein 2
• Calculated Molecular Weight: 386 aa, 42 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC016979
• Gene Symbol: NECAB2
• Gene ID (NCBI): 54550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z6G3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NECAB2 (N-terminal EF-hand calcium-binding protein 2) is a neuronal calcium sensor that functions as a signaling scaffold to modulate G protein-coupled receptor activity. It binds calcium via its N-terminal EF-hand domain and interacts directly with the adenosine A2A receptor (ADORA2A) and metabotropic glutamate receptor 5 (mGluR5), regulating their cell surface expression, internalization, and downstream signaling such as ERK1/2 activation . The protein is predominantly expressed in the brain and spinal cord, where it plays a critical role in pain signaling; its loss in dorsal root ganglia and spinal interneurons significantly accelerates recovery from inflammatory pain hypersensitivity in mice.