Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12145-1-AP targets NEIL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2793 Product name: Recombinant human NEIL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-390 aa of BC010876 Sequence: MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGRGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSAS Predict reactive species |
| Full Name | nei endonuclease VIII-like 1 (E. coli) |
| Calculated Molecular Weight | 390 aa, 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC010876 |
| Gene Symbol | NEIL1 |
| Gene ID (NCBI) | 79661 |
| RRID | AB_2251228 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96FI4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)
Read more about this antibody on the blog: http://blog.ptglab.com/index.php/proteintechs-anti-neil3-antibody-used-investigate-mechanisms-of-dna-repair/?preview=true
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NEIL1 antibody 12145-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NEIL1 antibody (Cat# 12145-1-AP) has 12 citations spanning journals including Nature, Nature Communications, JACS, Free Radical Biology and Medicine, Journal of Biological Chemistry, Frontiers in Cell and Developmental Biology, Scientific Reports, Frontiers in Oncology, DNA Repair, Toxicology and Applied Pharmacology, Biomedical Research International, and Environmental and Molecular Mutagenesis. Research fields encompass DNA base excision repair, DNA glycosylase function, oxidative DNA damage, mitochondrial biology, cancer (colorectal and cervical), and toxicology.
| Species | Application | Title |
|---|---|---|
J Am Chem Soc DNA-Protein Cross-Linking Sequencing for Genome-Wide Mapping of Thymidine Glycol. | ||
Free Radic Biol Med NEIL1: the second DNA glycosylase involved in action-at-a-distance mutations induced by 8-oxo-7,8-dihydroguanine
| ||
Front Cell Dev Biol Novel interaction interfaces mediate the interaction between the NEIL1 DNA glycosylase and mitochondrial transcription factor A | ||
Sci Rep Cervical carcinoma risk associate with genetic polymorphisms of NEIL2 gene in Chinese population and its significance as predictive biomarker. |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer reported that the antibody is working well for Western Blot applications, using a 1:1000 dilution on liver tissue. Overall, the feedback is positive, confirming reliable performance for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-26-2025) | WORKING WELL FOR wb APPLICATION
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