NEIL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NEIL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 12145-1-AP

Synonyms

DNA glycosylase/AP lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII like 1, FPG1, hFPG1, NEH1, Nei homolog 1, Nei like protein 1, NEI1, NEIL1

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Validation Data Gallery

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  WB analysis using 12145-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12145-1-AP (NEIL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below

Product Information

12145-1-AP targets NEIL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEIL1 fusion protein Ag2793
• Full Name: nei endonuclease VIII-like 1 (E. coli)
• Calculated Molecular Weight: 390 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC010876
• Gene Symbol: NEIL1
• Gene ID (NCBI): 79661
• RRID: AB_2251228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEIL1, also named as NEH1 and FPG1, belongs to the FPG family. It is involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. NEIL1 acts as DNA glycosylase that recognizes and removes damaged bases. It has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. NEIL1 has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. It cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. NEIL1 has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. The increased BER activity of NEILs may represent an adaptive response against ROS-induced DNA damage resulting from aniline exposure, and could be an important mechanism for the removal of oxidative DNA lesions. (PMID:21145906)

Read more about this antibody on the blog: http://blog.ptglab.com/index.php/proteintechs-anti-neil3-antibody-used-investigate-mechanisms-of-dna-repair/?preview=true

Publications

Species	Application	Title
human	WB	Nature; Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.; Authors - Shangming Tang
human	WB	J Am Chem Soc; DNA-Protein Cross-Linking Sequencing for Genome-Wide Mapping of Thymidine Glycol.; Authors - Feng Tang
human	WB	Nat Commun; Validating the concept of mutational signatures with isogenic cell models.; Authors - Xueqing Zou
human	WB	Front Cell Dev Biol; Novel interaction interfaces mediate the interaction between the NEIL1 DNA glycosylase and mitochondrial transcription factor A; Authors - Nidhi Sharma
human	WB	Front Oncol; Mono- and biallelic germline variants of DNA glycosylase genes in colon adenomatous polyposis families from two continents; Authors - Alisa Petriina Olkinuora
human	WB	Sci Rep; Cervical carcinoma risk associate with genetic polymorphisms of NEIL2 gene in Chinese population and its significance as predictive biomarker.; Authors - Feng Ye