NEIL1 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP00380-3

  Sandwich ELISA standard curve of MP00380-3, Human NEIL1 Recombinant Matched Antibody Pair - PBS only. 83376-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2793. 83376-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP00380-3

  The mean NEIL1 concentration was determined to be 12.20 ng/mL in HeLa cell extract based on a 2.80 mg/mL extract load.

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  Cytometric bead array standard curve of MP00380-3

  Cytometric bead array standard curve of MP00380-3, NEIL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83376-6-PBS. Detection antibody: 83376-1-PBS. Standard: Ag2793. Range: 1.56-100 ng/mL.

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  Affinity and Kinetic Characterization of 83376-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83376-1-PBS against Human NEIL1 was performed. The affinity constant is 4.54 nM.

NEIL1 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83376-1-PBS

Clone No.240322A8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

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Product Information

83376-1-PBS targets NEIL1 as part of a matched antibody pair:

MP00380-3: 83376-6-PBS capture and 83376-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2793

  Product name: Recombinant human NEIL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-390 aa of BC010876

  Sequence: MPEGPELHLASQFVNEACRALVFGGCVEKSSVSRNPEVPFESSAYRISASARGKELRLILSPLPGAQPQQEPLALVFRFGMSGSFQLVPREELPRHAHLRFYTAPPGPRLALCFVDIRRFGRWDLGGKWQPGRGPCVLQEYQQFRENVLRNLADKAFDRPICEALLDQRFFNGIGNYLRAEILYRLKIPPFEKARSVLEALQQHRPSPELTLSQKIRTKLQNPDLLELCHSVPKEVVQLGGRGYGSESGEEDFAAFRAWLRCYGMPGMSSLQDRHGRTIWFQGDPGPLAPKGRKSRKKKSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPEGTSAS

• Full Name: nei endonuclease VIII-like 1 (E. coli)
• Calculated Molecular Weight: 390 aa, 44 kDa
• GenBank Accession Number: BC010876
• Gene Symbol: NEIL1
• Gene ID (NCBI): 79661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96FI4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.