NEIL1 Recombinant antibody, PBS Only (Detector)

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  IHC staining of mouse spleen using 83376-3-RR (same clone as 83376-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83376-3-RR (NEIL1 antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83376-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83376-3-RR (same clone as 83376-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NEIL1 antibody (83376-3-RR, Clone: 240322B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83376-3-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83376-3-RR (same clone as 83376-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Neil1 Recombinant Antibody (83376-3-RR, Clone:240322B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83376-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00380-2

  Cytometric bead array standard curve of MP00380-2, NEIL1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83376-4-PBS. Detection antibody: 83376-3-PBS. Standard: Ag2793. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83376-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83376-3-PBS against Human NEIL1 was performed. The affinity constant is below 1 pM.

NEIL1 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83376-3-PBS

Clone No.240322B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

NEI L1, Endonuclease VIII-like 1, Endonuclease 8-like 1, EC:3.2.2.-, DNA-(apurinic or apyrimidinic site) lyase Neil1

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Product Information

83376-3-PBS targets NEIL1 as part of a matched antibody pair:

MP00380-2: 83376-4-PBS capture and 83376-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NEIL1 fusion protein Ag2793
• Full Name: nei endonuclease VIII-like 1 (E. coli)
• Calculated Molecular Weight: 390 aa, 44 kDa
• GenBank Accession Number: BC010876
• Gene Symbol: NEIL1
• Gene ID (NCBI): 79661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96FI4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.