NEK11 Polyclonal antibody

NEK11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12710-1-AP

Synonyms

NEK11, NimA related protein kinase 11

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Validation Data Gallery

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  WB analysis of HeLa using 12710-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12710-1-AP (NEK11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12710-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12710-1-AP (NEK11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12710-1-AP targets NEK11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEK11 fusion protein Ag3392
• Full Name: NIMA (never in mitosis gene a)- related kinase 11
• Calculated Molecular Weight: 54 kDa, 74 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC028587
• Gene Symbol: NEK11
• Gene ID (NCBI): 79858
• RRID: AB_2150345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEK11(Serine/threonine-protein kinase) belongs to the NIMA family of kinases, which are involved in DNA replication and genotoxic stress responses.It controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.It has 4 isoforms produced by alternative splicing.