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  • KD/KO Validated

NEK2 Polyclonal antibody

NEK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 24171-1-AP


HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24171-1-AP is NEK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK2 fusion protein Ag21476
Full Name NIMA (never in mitosis gene a)-related kinase 2
Calculated molecular weight 52 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC043502
Gene symbol NEK2
Gene ID (NCBI) 4751
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Product Specific Protocols
WB protocol for NEK2 antibody 24171-1-APDownload protocol
IHC protocol for NEK2 antibody 24171-1-APDownload protocol
IF protocol for NEK2 antibody 24171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Biol Sci

NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.

Authors - Rui Bai
  • KD Validated

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang

Nat Commun

NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.

Authors - Xiaozhen Zhang
  • KD Validated