|Positive WB detected in
|Positive IHC detected in
|mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 24171-1-AP is NEK2 Fusion Protein expressed in E. coli.
|Host / Isotype
|Rabbit / IgG
|NEK2 fusion protein Ag21476
|NIMA (never in mitosis gene a)-related kinase 2
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.
RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.
Biochim Biophys Acta Mol Cell Res
NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis
Int J Biol Sci
NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.
NEK2 is associated with poor prognosis of clear cell renal cell carcinoma and promotes tumor cell growth and metastasis
Circular forms of dedicator of cytokinesis 1 promotes breast cancer progression by derepressing never in mitosis related kinase 2 via sponging miR-128-3p
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah-Eve (Verified Customer) (06-02-2023)
Works very well WB dilution 1:1000 and for immunofluorescence of paraffin embedded tissue