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  • KD/KO Validated

NEK2 Polyclonal antibody

NEK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24171-1-AP

Synonyms

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1



Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24171-1-AP is NEK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK2 fusion protein Ag21476
Full Name NIMA (never in mitosis gene a)-related kinase 2
Calculated molecular weight 52 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC043502
Gene symbol NEK2
Gene ID (NCBI) 4751
RRIDAB_2687577
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NEK2 antibody 24171-1-APDownload protocol
IHC protocol for NEK2 antibody 24171-1-APDownload protocol
IF protocol for NEK2 antibody 24171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.

Authors - Xiaozhen Zhang
  • KD Validated
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
mouse,humanIHC,WB

Biochim Biophys Acta Mol Cell Res

NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis

Authors - Youhua Peng
  • KD Validated
human,mouseIHC

Int J Biol Sci

NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.

Authors - Rui Bai
  • KD Validated
humanIHC,WB

Gene

NEK2 is associated with poor prognosis of clear cell renal cell carcinoma and promotes tumor cell growth and metastasis

Authors - Xiaoli Feng
  • KD Validated
humanWB

Environ Toxicol

Circular forms of dedicator of cytokinesis 1 promotes breast cancer progression by derepressing never in mitosis related kinase 2 via sponging miR-128-3p

Authors - Zhaoxian Ni

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works very well WB dilution 1:1000 and for immunofluorescence of paraffin embedded tissue

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: Human pulmonary artery smooth muscle cells