Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24171-1-AP targets NEK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21476 Product name: Recombinant human NEK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-445 aa of BC043502 Sequence: MPSRAEDYEVLYTIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEAEKQMLVSEVNLLRELKHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCALMPPFTAFSQKELAGKIREGKFRRIPYRYSDELNEIITRMLNLKDYHRPSVEEILENPLIADLVADEQRRNLERRGRQLGEPEKSQDSSPVLSELKLKEIQLQERERALKAREERLEQKEQELCVRERLAEDKLARAENLLKNYSLLKERKFLSLASNPELLNLPSSVIKKKVHFSGESKENIMRSENSESQLTSKSKCKDLKKRLHAAQLRAQALSDIEKNYQLKSRQILGMR Predict reactive species |
| Full Name | NIMA (never in mitosis gene a)-related kinase 2 |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC043502 |
| Gene Symbol | NEK2 |
| Gene ID (NCBI) | 4751 |
| RRID | AB_2687577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NEK2 antibody 24171-1-AP | Download protocol |
| IHC protocol for NEK2 antibody 24171-1-AP | Download protocol |
| WB protocol for NEK2 antibody 24171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NEK2 antibody (Cat# 24171-1-AP) has 15 citations spanning journals including Cell Metabolism, Nature Communications, Current Biology, International Journal of Biological Sciences, Scientific Reports, Carcinogenesis, Molecular Carcinogenesis, Oncology Reports, Medicine & Oncology, International Immunopharmacology, Gene, Birth Defects Research, Environ Toxicol, BBA – Molecular Cell Research, and Discover Oncology. Research fields cover oncology (pancreatic, lung, breast, colorectal, renal, esophageal, gallbladder, oral, and osteosarcoma), immunology, cell biology, thrombosis, dermatology, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Cell Metab Targeting gut microbial nitrogen recycling and cellular uptake of ammonium to improve bortezomib resistance in multiple myeloma | ||
Nat Commun NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.
| ||
Int J Biol Sci NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.
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Curr Biol RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. | ||
Int Immunopharmacol KLF15 regulates macrophage polarization patterns in deep vein thrombosis | ||
Sci Rep NEK2 promotes oral squamous cell carcinoma progression and serves as a diagnostic and therapeutic target.
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Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer reported excellent performance in both Western Blot (at a 1:1000 dilution) and immunofluorescence on paraffin-embedded tissue, using human pulmonary artery smooth muscle cells. Overall, the feedback highlights reliable and strong results across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah-Eve (Verified Customer) (06-02-2023) | Works very well WB dilution 1:1000 and for immunofluorescence of paraffin embedded tissue
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