NEK2 Monoclonal antibody

NEK2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3D7B4

Cat no : 66632-1-Ig

Synonyms

HSPK 21, HsPK21, NEK2, NEK2A, NimA like protein kinase 1, NimA related protein kinase 2, NLK1



Tested Applications

Positive WB detected inHuman peripheral blood leukocyte, THP-1 cells, K-562 cells, HL-60 cells, Jurkat cells, RAW 264.7 cells, rat thymus tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66632-1-Ig targets NEK2 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Reactivity Human, Mouse , Rat
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEK2 fusion protein Ag21476
Full Name NIMA (never in mitosis gene a)-related kinase 2
Calculated molecular weight 52 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC043502
Gene symbol NEK2
Gene ID (NCBI) 4751
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NEK2 antibody 66632-1-IgDownload protocol
IHC protocol for NEK2 antibody 66632-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Toxicol Lett

Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.

Authors - Momoka Ota