NEK4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84654-4-RR (same clone as 84654-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84654-4-RR (NEK4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84654-4-RR (NEK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 84654-4-RR (same clone as 84654-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using NEK4 antibody (84654-4-RR, Clone: 242081B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84654-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84654-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84654-4-RR against Human NEK4 were performed. The affinity constant is 92.8 pM.

NEK4 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84654-4-PBS

Clone No.242081B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

242081B9, NimA related protein kinase 4, NRK2, pp12301, STK2

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Product Information

84654-4-PBS targets NEK4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34929

  Product name: Recombinant human NEK4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 429-779 aa of BC063044

  Sequence: SSDIVTGEKNEPVKPLQPLIKEQKPKDQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAFQESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSSSGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKGQTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMPGSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVSVSLTVSRCLCYR

• Full Name: NIMA (never in mitosis gene a)-related kinase 4
• Calculated Molecular Weight: 781 aa, 88 kDa
• Observed Molecular Weight: 95-100 kDa
• GenBank Accession Number: BC063044
• Gene Symbol: NEK4
• Gene ID (NCBI): 6787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P51957
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Neks (NIMA-related kinases) are a group of serine-threonine kinases that are related to NIMA, their ortholog from Aspergillus nidulans, which is essential for cells to enter in mitosis. NEK4 was initially identified as STK2, from serine/threonine kinase 2, in a study using a kinase specific cDNA library from human breast cancer tumors or breast cancer cells.