NEK6 Polyclonal antibody

• Featured Product
• KD/KO Validated

NEK6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10378-1-AP

Synonyms

Protein kinase SID6-1512, Protein kinase SID6 1512, NimA-related protein kinase 6, NimA related protein kinase 6, Never in mitosis A-related kinase 6

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Validation Data Gallery

• 

  WB analysis using 10378-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10378-1-AP

  WB result of NEK6 antibody (10378-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK6 transfected HEK-293 cells.

• 

  WB analysis of HT-1080 using 10378-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10378-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10378-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10378-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10378-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 10378-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10378-1-AP (NEK6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HT-1080 cells, HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

10378-1-AP targets NEK6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEK6 fusion protein Ag0266
• Full Name: NIMA (never in mitosis gene a)-related kinase 6
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-45 kDa
• GenBank Accession Number: BC000101
• Gene Symbol: NEK6
• Gene ID (NCBI): 10783
• RRID: AB_2251272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Aspergillus nidulans 'never in mitosis A' (NIMA) is a serine/ threonine kinase that controls initiation of mitosis, whereas its inactivation is necessary for mitotic exit. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. Human NIMA-related kinase 6 (NEK6, synonym: SID6-1512) is comprised of 338 amino acids and shows both nuclear and cytoplasmic localizations in HeLa cells. NEK6 is required for mitotic progression of human cells. NEK6 is phosphorylated and activated during M phase. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis. Recombinant human NEK6 protein produced in insect cells effectively phosphorylates histones H1 and H3, but not casein. Thus, these results suggest that, unlike other mammalian NIMA-related kinases, NEK6 is a mitotic histone kinase which regulates chromatin condensation in mammalian cells. NEK6 transcripts are ubiquitously expressed with the highest expression found in the heart and skeletal muscle, and the hNek6 gene is localized to human chromosome 9q33-34. NEK6 has four isoforms with MW 35-40 kDa.

Publications

Species	Application	Title
human	WB	Front Mol Biosci; LncRNA FAM13A-AS1 Promotes Renal Carcinoma Tumorigenesis Through Sponging miR-141-3p to Upregulate NEK6 Expression.; Authors - Xin Jun Wang
human	WB	J Oncol; MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells.; Authors - Zhongshi Hong
human	WB,IF	Int J Gen Med; NEK6 Promotes the Progression of Osteosarcoma Through Activating STAT3 Signaling Pathway by Down-Regulation of miR-26a-5p; Authors - Min Zhu
human	WB	Cancers (Basel); Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus; Authors - Lei Chen