Home
Products
Primary Antibodies
NELF-A Polyclonal antibody, PBS Only (10456-1-PBS)

Filter: AllWBIPIF/ICC

WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.
WB analysis using 10456-1-AP (same clone as 10456-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 10456-1-AP (same clone as 10456-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 10456-1-AP (same clone as 10456-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 10456-1-AP (same clone as 10456-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 10456-1-AP (same clone as 10456-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 10456-1-AP (NELF-A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 10456-1-AP (same clone as 10456-1-PBS)
IP result of anti-NELF-A (IP:10456-1-AP, 4ug; Detection:10456-1-AP 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 10456-1-AP (same clone as 10456-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NELF-A antibody (10456-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10456-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

NELF-A Polyclonal antibody, PBS Only

Name: NELF-A Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10456-1-PBS

NELF-A Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 10456-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

WHSC2, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, NELF A

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

10456-1-PBS targets NELF-A in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0708 Product name: Recombinant human NELF-A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-314 aa of BC002764 Sequence: ESDTGLWLHNKLGATDELWAPPSIASLLTAAVIDNIRLCFHGLSSAVKLKLLLGTLHLPRRTVDEMKGALMEIIQLASLDSDPWVLMVADILKSFPDTGSLNLELEEQNPNVQDILGELREKVGECEASAMLPLECQYLNKNALTTLAGPLTPPVKHFQLKRKPKSATLRAELLQKSTETAQQLKRSAGVPFHAKGRGLLRKMDTTTPLKGIPKQAPFRSPTAPSVFSPTGNRTPIPPSRTLLRKERGVKLLDISELDMVGAGREAKRRRKTLDAEVVEKPAKEETVVENATPDYAAGLVSTQKLGSLN Predict reactive species
Full Name	Wolf-Hirschhorn syndrome candidate 2
Calculated Molecular Weight	57 kDa
Observed Molecular Weight	57 kDa
GenBank Accession Number	BC002764
Gene Symbol	NELFA
Gene ID (NCBI)	7469
RRID	AB_2216327
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H3P2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

WHSC2, also known as NELF-A, is a component of the Negative ELongation Factor complex, which inhibits RNA polymerase II progression early during elongation (a process referred to as 'promoter proximal pausing'), thereby altering expression of its target genes in a positive and negative manner [PMID:19245807]. This complex acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 and SPT5, to cause transcriptional pausing of RNA polymerase II[PMID:12612062].