NELL2 Polyclonal antibody

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• NELL2 Polyclonal antibody (11268-1-AP)

NELL2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11268-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

NEL like protein 2, Nel related protein 2, NEL-like protein 2, Nel-related protein 2, NRP2

• 

  WB analysis using 11268-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11268-1-AP (NELL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 11268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11268-1-AP (NELL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 11268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11268-1-AP (NELL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: fetal human brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11268-1-AP targets NELL2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1793

  Product name: Recombinant human NELL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 443-740 aa of BC020544

  Sequence: ECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSDGFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENPTVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVE

• Full Name: NEL-like 2 (chicken)
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC020544
• Gene Symbol: NELL2
• Gene ID (NCBI): 4753
• RRID: AB_10638004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99435
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

NEL2 Polyclonal antibody (Cat# 11268-1-AP) has 12 citations across journals including Signal Transduction and Targeted Therapy, Nature Communications, Communications Biology, Reproductive Biology and Endocrinology, Oncology Reports, Frontiers in Molecular Biosciences, International Journal of Medical Sciences, Chemistry, Neuroscience, Frontiers in Neurology, Discovery Oncology, and Cell and Development. Associated research fields span colorectal cancer, male reproductive biology, renal cell carcinoma, lymphoma, esophageal cancer, neurotoxicity, and chromatin accessibility.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Human		Signal Transduct Target Ther; Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis.; Authors - Xiaobo Zheng
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
human	IF	Commun Biol; Single-cell profiling of the microenvironment in human bone metastatic renal cell carcinoma; Authors - Fen Ma
human	WB	Reprod Biol Endocrinol; Expression of NELL2/NICOL-ROS1 lumicrine signaling-related molecules in the human male reproductive tract; Authors - Daiji Kiyozumi
human	IHC	Oncol Rep; microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma.; Authors - Jianhong Fu
human	WB,IHC	Front Mol Biosci; An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.; Authors - Hongpan Zhang