GNAS Polyclonal antibody

• Featured Product
• KD/KO Validated

GNAS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 10150-2-AP

Synonyms

AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH

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Validation Data Gallery

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  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10150-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10150-2-AP

  IP result of anti-GNAS (IP:10150-2-AP, 4ug; Detection:10150-2-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse brain using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10150-2-AP (GNAS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10150-2-AP (GNAS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10150-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GNAS antibody (10150-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594-phalloidine (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue, rat brain tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse brain tissue, human pancreas tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10150-2-AP targets GNAS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNAS fusion protein Ag0197
• Full Name: GNAS complex locus
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002722
• Gene Symbol: GNAS
• Gene ID (NCBI): 2778
• RRID: AB_2111668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Publications

Species	Application	Title
mouse	WB	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	WB	Cell Mol Life Sci; HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.; Authors - Gemoll Timo T
human	WB,IF	Cell Mol Biol Lett; GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.; Authors - Hongda Ding
mouse	WB,IF	Am J Physiol Renal Physiol; Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.; Authors - Lele Liu ; KD Validated
human	WB	Tumour Biol; A novel GNAS-mutated human induced pluripotent stem cell model for understanding GNAS-mutated tumors.; Authors - Katsuhito Watanabe ; KO Validated
human,mouse	WB	Nat Commun; Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity; Authors - Yazhong Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (10-16-2023)

Works well.

• Applications: Western Blot