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  • KD/KO Validated

GNAS Polyclonal antibody

GNAS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10150-2-AP

Synonyms

AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH



Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue, rat brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue, human pancreas tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10150-2-AP targets GNAS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNAS fusion protein Ag0197
Full Name GNAS complex locus
Calculated molecular weight 45 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002722
Gene symbol GNAS
Gene ID (NCBI) 2778
RRIDAB_2111668
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Protocols

Product Specific Protocols
WB protocol for GNAS antibody 10150-2-APDownload protocol
IHC protocol for GNAS antibody 10150-2-APDownload protocol
IF protocol for GNAS antibody 10150-2-APDownload protocol
IP protocol for GNAS antibody 10150-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang
humanWB

Cell Mol Life Sci

HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.

Authors - Gemoll Timo T
humanWB,IF

Cell Mol Biol Lett

GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.

Authors - Hongda Ding
mouseWB,IF

Am J Physiol Renal Physiol

Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.

Authors - Lele Liu
  • KD Validated
humanWB

Tumour Biol

A novel GNAS-mutated human induced pluripotent stem cell model for understanding GNAS-mutated tumors.

Authors - Katsuhito Watanabe
  • KO Validated
human,mouseWB

Nat Commun

Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity

Authors - Yazhong Cui

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Parijat (Verified Customer) (10-16-2023)

Works well.

  • Applications: Western Blot