|Positive WB detected in||Jurkat cells, mouse liver tissue, MCF-7 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human pancreas tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10150-2-AP targets GNAS in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GNAS fusion protein Ag0197|
|Full Name||GNAS complex locus|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC002722|
|Gene ID (NCBI)||2778|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.
Am J Physiol Renal Physiol
Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.
A novel GNAS-mutated human induced pluripotent stem cell model for understanding GNAS-mutated tumors.
Cell Mol Biol Lett
GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.
Cell Mol Life Sci
HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.
Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.