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  • KD/KO Validated

GNAS Polyclonal antibody

GNAS Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10150-2-AP

Synonyms

AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH



Tested Applications

Positive WB detected inJurkat cells, mouse liver tissue, MCF-7 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman pancreas tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10150-2-AP targets GNAS in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNAS fusion protein Ag0197
Full Name GNAS complex locus
Calculated molecular weight 45 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002722
Gene symbol GNAS
Gene ID (NCBI) 2778
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Protocols

Product Specific Protocols
WB protocol for GNAS antibody 10150-2-APDownload protocol
IHC protocol for GNAS antibody 10150-2-APDownload protocol
IP protocol for GNAS antibody 10150-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Am J Physiol Renal Physiol

Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.

Authors - Lele Liu
  • KD Validated
humanWB

Tumour Biol

A novel GNAS-mutated human induced pluripotent stem cell model for understanding GNAS-mutated tumors.

Authors - Katsuhito Watanabe
  • KO Validated
humanWB,IF

Cell Mol Biol Lett

GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.

Authors - Hongda Ding
humanWB

Cell Mol Life Sci

HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.

Authors - Gemoll Timo T
mouseWB

Elife

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Authors - Dao-Lai Zhang