Validation Data Gallery
|Positive WB detected in
|Jurkat cells, mouse brain tissue, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue, rat brain tissue
|Positive IP detected in
|mouse liver tissue
|Positive IHC detected in
|mouse brain tissue, human pancreas tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:2000-1:12000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:200-1:800
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10150-2-AP targets GNAS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|GNAS fusion protein Ag0197
|GNAS complex locus
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.
|Product Specific Protocols
|WB protocol for GNAS antibody 10150-2-AP
|IHC protocol for GNAS antibody 10150-2-AP
|IF protocol for GNAS antibody 10150-2-AP
|IP protocol for GNAS antibody 10150-2-AP
|Click here to view our Standard Protocols
Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.
Cell Mol Life Sci
HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.
Cell Mol Biol Lett
GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.
Am J Physiol Renal Physiol
Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.
A novel GNAS-mutated human induced pluripotent stem cell model for understanding GNAS-mutated tumors.
Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity
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Parijat (Verified Customer) (10-16-2023)