GNAS Polyclonal antibody

• 

  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10150-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10150-2-AP

  IP result of anti-GNAS (IP:10150-2-AP, 4ug; Detection:10150-2-AP 1:5000) with mouse liver tissue lysate 2240 ug.

• 

  IHC staining of mouse brain using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10150-2-AP (GNAS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10150-2-AP (GNAS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 10150-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GNAS antibody (10150-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594-phalloidine (red).

• 

  IF Staining of MCF-7 using 10150-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (10150-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

GNAS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10150-2-AP

Publications(17)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

---

Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue, rat brain tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human colon tissue, human pancreas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

10150-2-AP targets GNAS in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0197

  Product name: Recombinant human NESP55,GNAS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 230-394 aa of BC002722

  Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

• Full Name: GNAS complex locus
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002722
• Gene Symbol: GNAS
• Gene ID (NCBI): 2778
• RRID: AB_2111668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95467
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP	Cancer Res; PRKDC Induces Chemoresistance in Osteosarcoma by Recruiting GDE2 to Stabilize GNAS and Activate AKT; Authors - Wenchao Zhang ; KD Validated
mouse	WB	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	WB	Cell Mol Life Sci; HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.; Authors - Gemoll Timo T
mouse	WB,IF	Sci Rep; Sex-specific transgenerational effects on murine thyroid gland imposed by ancestral exposure to neonicotinoid thiacloprid; Authors - Mariam Diba Lahmidi
human	WB,IF	Cell Mol Biol Lett; GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.; Authors - Hongda Ding
mouse	WB,IF	Am J Physiol Renal Physiol; Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.; Authors - Lele Liu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Parijat (Verified Customer) (10-16-2023)

Works well.

• Applications: Western Blot