NESP55,GNAS Recombinant monoclonal antibody

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• NESP55,GNAS Recombinant monoclonal antibody (81802-2-RR)

• KD/KO Validated

NESP55,GNAS Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 81802-2-RR

Clone No.251463D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

GNAS, GNAS1, GPIPIRRH peptide, LHAL tetrapeptide, NESP55

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  WB analysis using 81802-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81802-2-RR (NESP55,GNAS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 81802-2-RR

  WB result of NESP55,GNAS antibody (81802-2-RR; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NESP55,GNAS transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 81802-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NESP55,GNAS antibody (81802-2-RR, Clone: 251463D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 81802-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81802-2-RR against Human NESP55,GNAS were performed. The affinity constant is below 1 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, MCF-7 cells, Jurkat cells, MCGF-7 cells, mouse liver tissue, rat liver tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81802-2-RR targets NESP55,GNAS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0197

  Product name: Recombinant human NESP55,GNAS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 230-394 aa of BC002722

  Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

• Full Name: GNAS complex locus
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002722
• Gene Symbol: GNAS
• Gene ID (NCBI): 2778
• RRID: AB_3743396
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P63092
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The near two bands represent two different isoforms. The expression profile/molecular weight observed are consistent with what has been described in the literature (PMID : 3258290).