NEU2 Polyclonal antibody

NEU2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24523-1-AP

Synonyms

Cytosolic sialidase, N acetyl alpha neuraminidase 2, NEU2, SIAL2, Sialidase 2

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Validation Data Gallery

• 

  WB analysis using 24523-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 24523-1-AP (NEU2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 24523-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24523-1-AP (NEU2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24523-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24523-1-AP (NEU2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-12 cells, HepG2 cells, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

24523-1-AP targets NEU2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEU2 fusion protein Ag17386
• Full Name: sialidase 2 (cytosolic sialidase)
• Calculated Molecular Weight: 380 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC107053
• Gene Symbol: NEU2
• Gene ID (NCBI): 4759
• RRID: AB_2879588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Exp Lung Res; Changes in lung sialidases in male and female mice after bleomycin aspiration; Authors - Darrell Pilling