NEU3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NEU3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 27879-1-AP

Synonyms

cDNA FLJ78026, NEU3, NEU3 protein, SIAL3

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Validation Data Gallery

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  WB analysis using 27879-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27879-1-AP (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 27879-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27879-1-AP (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 27879-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27879-1-AP (NEU3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 27879-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27879-1-AP (NEU3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 27879-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using NEU3 antibody (27879-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells, THP-1 cells
• Positive IHC detected in: mouse testis tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

27879-1-AP targets NEU3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEU3 fusion protein Ag27259
• Full Name: sialidase 3 (membrane sialidase)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48, 52 kDa
• GenBank Accession Number: NM_006656.5
• Gene Symbol: NEU3
• Gene ID (NCBI): 10825
• RRID: AB_2881002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	JCI Insight; The mRNA binding protein DDX3 mediates TGF-β1 upregulation of translation and promotes pulmonary fibrosis; Authors - Wensheng Chen
human,mouse	WB,IHC	Food Funct; Polyphenol from millet bran increases the sensitivity of colorectal cancer cells to oxaliplatin by blocking the ganglioside GM3 catabolism.; Authors - Xiaoli Zhang ; KD Validated
mouse	IF	Exp Lung Res; Changes in lung sialidases in male and female mice after bleomycin aspiration; Authors - Darrell Pilling

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Prem (Verified Customer) (10-21-2022)

In the data sheet, the clear band of Neu3 was shown but in my case there are multiple bands. I dont know it might be because of different lot of antibody. it did not work for my western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1;2000
• Cell Tissue Type: MOUSE liver tissue

NEU3 Antibody Western Blot validation (1;2000 dilution) in MOUSE liver tissue (Cat no:27879-1-AP)