• Featured Product
  • KD/KO Validated

NEU3 Polyclonal antibody

NEU3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27879-1-AP

Synonyms

cDNA FLJ78026, NEU3, NEU3 protein, SIAL3



Tested Applications

Positive WB detected inHEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells, THP-1 cells
Positive IHC detected inmouse testis tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27879-1-AP targets NEU3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEU3 fusion protein Ag27259
Full Name sialidase 3 (membrane sialidase)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48, 52 kDa
GenBank Accession NumberNM_006656.5
Gene Symbol NEU3
Gene ID (NCBI) 10825
RRIDAB_2881002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NEU3 antibody 27879-1-APDownload protocol
IHC protocol for NEU3 antibody 27879-1-APDownload protocol
IF protocol for NEU3 antibody 27879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

JCI Insight

The mRNA binding protein DDX3 mediates TGF-β1 upregulation of translation and promotes pulmonary fibrosis

Authors - Wensheng Chen
human,mouseWB,IHC

Food Funct

Polyphenol from millet bran increases the sensitivity of colorectal cancer cells to oxaliplatin by blocking the ganglioside GM3 catabolism.

Authors - Xiaoli Zhang
  • KD Validated
mouseIF

Exp Lung Res

Changes in lung sialidases in male and female mice after bleomycin aspiration

Authors - Darrell Pilling
human,mouseWB,IHC

PLoS One

Inhibition of CCl4-induced liver inflammation and fibrosis by a NEU3 inhibitor

Authors - Darrell Pilling

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Prem (Verified Customer) (10-21-2022)

In the data sheet, the clear band of Neu3 was shown but in my case there are multiple bands. I dont know it might be because of different lot of antibody. it did not work for my western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1;2000
  • Cell Tissue Type: MOUSE liver tissue
NEU3 Antibody Western Blot validation (1;2000 dilution) in MOUSE liver tissue (Cat no:27879-1-AP)