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NEU3 Polyclonal antibody
NEU3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 27879-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells, THP-1 cells |
Positive IHC detected in | mouse testis tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse small intestine tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
27879-1-AP targets NEU3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NEU3 fusion protein Ag27259 |
Full Name | sialidase 3 (membrane sialidase) |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 48, 52 kDa |
GenBank Accession Number | NM_006656.5 |
Gene Symbol | NEU3 |
Gene ID (NCBI) | 10825 |
RRID | AB_2881002 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for NEU3 antibody 27879-1-AP | Download protocol |
IHC protocol for NEU3 antibody 27879-1-AP | Download protocol |
IF protocol for NEU3 antibody 27879-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
JCI Insight The mRNA binding protein DDX3 mediates TGF-β1 upregulation of translation and promotes pulmonary fibrosis | ||
Food Funct Polyphenol from millet bran increases the sensitivity of colorectal cancer cells to oxaliplatin by blocking the ganglioside GM3 catabolism.
| ||
Exp Lung Res Changes in lung sialidases in male and female mice after bleomycin aspiration | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Prem (Verified Customer) (10-21-2022) | In the data sheet, the clear band of Neu3 was shown but in my case there are multiple bands. I dont know it might be because of different lot of antibody. it did not work for my western blot.
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