NEURL2 Polyclonal antibody

NEURL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21263-1-AP

Synonyms

C20orf163, Neuralized like protein 2, NEURL2, OZZ, Ozz E3

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Validation Data Gallery

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  WB analysis of HL-60 using 21263-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21263-1-AP (NEURL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 21263-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21263-1-AP (NEURL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21263-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21263-1-AP (NEURL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 21263-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 21263-1-AP (NEURL2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 21263-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 21263-1-AP (NEURL2 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 21263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21263-1-AP (NEURL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21263-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21263-1-AP (NEURL2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells, human heart tissue
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21263-1-AP is NEURL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEURL2 fusion protein Ag15670
• Full Name: neuralized homolog 2 (Drosophila)
• Calculated Molecular Weight: 285 aa, 32 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC105935
• Gene Symbol: NEURL2
• Gene ID (NCBI): 140825
• RRID: AB_10755296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.