Filter: AllWBIHC

WB analysis of HL-60 using 21263-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 21263-1-AP (NEURL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 21263-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 21263-1-AP (NEURL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 21263-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 21263-1-AP (NEURL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 21263-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 21263-1-AP (NEURL2 Antibody) at dilution of 1:50.
IHC staining of human skeletal muscle using 21263-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 21263-1-AP (NEURL2 Antibody) at dilution of 1:50.
IHC staining of human heart using 21263-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21263-1-AP (NEURL2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 21263-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21263-1-AP (NEURL2 antibody) at dilution of 1:50 (under 40x lens).

NEURL2 Polyclonal antibody

Name: NEURL2 Polyclonal antibody
Brand: Proteintech
SKU: 21263-1-AP

Cat No. 21263-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C20orf163, Neuralized like protein 2, Neuralized-like protein 2, OZZ, Ozz E3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	HL-60 cells, human heart tissue
Positive IHC detected in	human skeletal muscle tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

21263-1-AP targets NEURL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15670 Product name: Recombinant human NEURL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC105935 Sequence: MAAASEPVDSGALWGLERPEPPPTRFHRVHGANIRVDPSGTRATRVESFAHGVCFSREPLAPGQVFLVEIEEKELGWCGHLRLGLTALDPASLAPVPEFSLPDLVNLGHTWVFAITRHHNRVPREGRPEAEAAAPSRPPTLLVEPYLRIEQFRIPRDRLVGRSRPGLYSHLLDQLYELNVLPPTARRSRLGVLFCPRPDGTADMHIIINGEDMGPSARGLPAAQPLYAVVDVFASTKSVRLVQLEYGLPSLQTLCRLVIQRSMVHRLAIDGLHLPKELKDFCKYE Predict reactive species
Full Name	neuralized homolog 2 (Drosophila)
Calculated Molecular Weight	285 aa, 32 kDa
Observed Molecular Weight	30-32 kDa
GenBank Accession Number	BC105935
Gene Symbol	NEURL2
Gene ID (NCBI)	140825
RRID	AB_10755296
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BR09
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.