NF45 Polyclonal antibody

NF45 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24572-1-AP

Synonyms

ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2

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Validation Data Gallery

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  WB analysis of K-562 using 24572-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP (NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24572-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP (NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24572-1-AP (NF45 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:200.

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  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:400.

• 

  IHC staining of human breast cancer using 24572-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:400.

• 

  IF Staining of HepG2 using 24572-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24572-1-AP (NF45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells
• Positive IHC detected in: human colon tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24572-1-AP targets NF45 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF45 fusion protein Ag21480
• Full Name: interleukin enhancer binding factor 2, 45kDa
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC000382
• Gene Symbol: ILF2
• Gene ID (NCBI): 3608
• RRID: AB_2879615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].