NF45 Monoclonal antibody, PBS Only

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  WB analysis using 67685-1-Ig (same clone as 67685-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67685-1-Ig (NF45 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 67685-1-Ig (same clone as 67685-1-PBS)

  IP result of anti-NF45 (IP:67685-1-Ig, 4ug; Detection:67685-1-Ig 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67685-1-Ig (NF45 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67685-1-Ig (same clone as 67685-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF45 antibody (67685-1-Ig, Clone: 1A3A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67685-1-PBS in a different storage buffer formulation.

NF45 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 67685-1-PBS

Clone No.1A3A2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ILF2, NF45, PRO3063

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Product Information

67685-1-PBS targets NF45 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30388

  Product name: Recombinant human NF45 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-390 aa of BC000382

  Sequence: MRGDRGRGRGGRFGSRGGPGGGFRPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEENTEEPPQGEEEESMETQE

• Full Name: interleukin enhancer binding factor 2, 45kDa
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC000382
• Gene Symbol: ILF2
• Gene ID (NCBI): 3608
• RRID: AB_2882878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12905
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].