Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, U2OS cells, COS-7 cells |
| Positive IHC detected in | mouse skeletal muscle tissue, human urothelial carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21713-1-AP targets NFAT5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16341 Product name: Recombinant human NFAT5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1231-1531 aa of BC146765 Sequence: MFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF Predict reactive species |
| Full Name | nuclear factor of activated T-cells 5, tonicity-responsive |
| Calculated Molecular Weight | 1531 aa, 166 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC146765 |
| Gene Symbol | NFAT5 |
| Gene ID (NCBI) | 10725 |
| RRID | AB_3085667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NFAT5 antibody 21713-1-AP | Download protocol |
| IHC protocol for NFAT5 antibody 21713-1-AP | Download protocol |
| WB protocol for NFAT5 antibody 21713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NFAT5 Polyclonal antibody (21713-1-AP) has 4 citations. They appear in Nature Metabolism, npj Science of Food, Microorganisms, and Annals of Surgical Oncology. Research fields span mitochondrial metabolism and tumour growth, high-salt diet–induced allergic rhinitis, coxsackievirus cardiac damage, and pancreatic cancer chemoresistance. Applications include WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Metab SLC6A6 imports taurine into mitochondria to sustain mitochondrial translation and tumour growth | ||
NPJ Sci Food High-salt diet aggravates allergic rhinitis through the NFAT5 signaling pathway. | ||
Microorganisms Cyclodextrin Counteracts Coxsackievirus-Induced Cardiac Damage by Protecting Desmosome Integrity and Suppressing Proinflammatory Cytokine Expression.
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Ann Surg Oncol Overexpression of Nuclear Factor of Activated T-cells (NFAT) Transcription Factors Drives Chemoresistance and Poor Outcomes in Pancreatic Cancer.
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Reviews
What customers say
The single available review rates the product 5 out of 5 stars. The reviewer reports that it works well for both Western Blot and Immunofluorescence, confirming reliable performance across these two applications. Overall, the feedback is positive, with no issues or limitations mentioned.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Parijat (Verified Customer) (10-16-2023) | Works well for western blot and IF
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