Tested Applications
| Positive WB detected in | Jurkat cells, U2OS cells, Daudi cells, Romas cells, Raji cells, A549 cells, MOLT-4 cells, Ramos cells, Karpas-422 cells |
| Positive IHC detected in | human urothelial carcinoma tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66963-1-Ig targets NFATC1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13014 Product name: Recombinant human NFATC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-351 aa of BC104753 Sequence: MPSTSFPVPSKFPLGPAAAVFGRGETLGPAPRAGGTMKSAEEEHYGYASSNVSPALPLPTAHSTLPAPCHNLQTSTPGIIPPADHPSGYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFHDVEVEDVLPSSKRSPSTATLSLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSPCVSPKTTDPEEGFPRGLGACTLLGSPRHSPSTSPRASVTEESWLGARSSRPASPCNKRKYSLNGRQPPYSPHHSPTPSPHGSPRVSVTDDSWLGNTTQYTSSAIVAAINALTTDSSLDLGDGVPVKSRKTTLEQPPSVALKVE Predict reactive species |
| Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 |
| Calculated Molecular Weight | 716aa,78 kDa; 943aa,101 kDa |
| Observed Molecular Weight | 101 kDa |
| GenBank Accession Number | BC104753 |
| Gene Symbol | NFATC1 |
| Gene ID (NCBI) | 4772 |
| RRID | AB_2882286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95644 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NFATC1 antibody 66963-1-Ig | Download protocol |
| WB protocol for NFATC1 antibody 66963-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
NFATC1 Monoclonal antibody (1E1B10), catalog number 66963-1-Ig, has 107 citations. Publications appear in journals including Signal Transduction and Targeted Therapy, Cell Reports, Cancer Letters, International Journal of Biological Macromolecules, Molecular Therapy, Journal of Bone and Mineral Research, iScience, Bone, and Frontiers in Immunology, among others. Research fields span osteoporosis and bone biology, oncology, immunology, periodontitis, rheumatoid arthritis, and muscle physiology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting N(1)-methyladenosine modification in osteoblasts through tRNA methyltransferase 10C reverses mitochondrial dysfunction and ameliorates osteoporosis. | ||
Cell Mol Immunol Glycogen synthase kinase 3 controls T-cell exhaustion by regulating NFAT activation | ||
Bioact Mater A parathyroid hormone related supramolecular peptide for multi-functionalized osteoregeneration | ||
J Extracell Vesicles Metastatic prostate cancer-derived extracellular vesicles facilitate osteoclastogenesis by transferring the CDCP1 protein | ||
Int J Oral Sci A 3D-printed molybdenum-containing scaffold exerts dual pro-osteogenic and anti-osteoclastogenic effects to facilitate alveolar bone repair | ||
Acta Pharm Sin B KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer |
