NFE2 Polyclonal antibody

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• NFE2 Polyclonal antibody (11089-1-AP)

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• KD/KO Validated

NFE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11089-1-AP Copied!

Publications (22)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

p45 NF E2, p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 11089-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 11089-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of K-562 using 11089-1-AP

  IP result of anti-NFE2 (IP:11089-1-AP, 4ug; Detection:11089-1-AP 1:500) with K-562 cells lysate 3440ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 11089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11089-1-AP (NFE2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 11089-1-AP

  Immunofluorescent analysis of HepG2 cells, using NFE2 antibody 11089-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, U-937 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11089-1-AP targets NFE2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1570

  Product name: Recombinant human NFE2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-373 aa of BC005044

  Sequence: MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD

• Full Name: nuclear factor (erythroid-derived 2), 45kDa
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC005044
• Gene Symbol: NFE2
• Gene ID (NCBI): 4778
• RRID: AB_2152928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16621
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. This is a rabbit polyclonal antibody raised against full-length NFE2 of human origin.

Publications

What published studies show

The anti-NFE2 antibody (Cat# 11089-1-AP) has 22 total citations. Citations appear in journals including Cell Research, Journal of the American College of Cardiology, Advanced Science, Cell Reports, International Journal of Biological Sciences, Life Sciences, International Immunopharmacology, and eLife. Research fields span thrombopoiesis and megakaryocyte differentiation, cardiac failure, erythroid differentiation, diabetic kidney fibrosis, pancreatic cancer metastasis, lung injury, ferroptosis, and prostate cancer progression.

Automatically generated from the 22 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cell Res; Cold exposure aggravates vein occlusion through non-shivering thermogenesis-induced thrombocytopoiesis; Authors - Sisi Xie
human	WB	J Am Coll Cardiol; Heme levels are increased in human failing hearts.; Authors - Khechaduri Arineh A
mouse	WB	Adv Sci (Weinh); Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.; Authors - Zhiyuan Lu ; KD Validated
human	WB	Int J Biol Sci; Genistin: A Novel Estrogen Analogue Targeting ERβ to Alleviate Thrombocytopenia; Authors - Xiaoqin Tang
human	WB,IF	J Pharm Anal; Oxymatrine, a novel TLR2 agonist, promotes megakaryopoiesis and thrombopoiesis through the STING/NF-κB pathway; Authors - Chengyang Ni
human	WB	Phytother Res; Harmine promotes megakaryocyte differentiation and thrombopoiesis by activating the Rac1/Cdc42/JNK pathway through a potential target of 5-HTR2A; Authors - Xiaoxi Liu