NFE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NFE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 11089-1-AP

Synonyms

p45 NF E2, p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2

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Validation Data Gallery

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11089-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11089-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11089-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP (NFE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11089-1-AP

  IP result of anti-NFE2 (IP:11089-1-AP, 4ug; Detection:11089-1-AP 1:500) with K-562 cells lysate 3440ug.

• 

  IHC staining of human colon cancer using 11089-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11089-1-AP (NFE2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11089-1-AP

  Immunofluorescent analysis of HepG2 cells, using NFE2 antibody 11089-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: K-562 cells, U-937 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

11089-1-AP targets NFE2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFE2 fusion protein Ag1570
• Full Name: nuclear factor (erythroid-derived 2), 45kDa
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC005044
• Gene Symbol: NFE2
• Gene ID (NCBI): 4778
• RRID: AB_2152928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. This is a rabbit polyclonal antibody raised against full-length NFE2 of human origin.

Publications

Species	Application	Title
human	WB	J Am Coll Cardiol; Heme levels are increased in human failing hearts.; Authors - Khechaduri Arineh A
mouse,human		Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin
human	WB	Life Sci; ERK and p38 MAPK inhibition controls NF-E2 degradation and profibrotic signaling in renal proximal tubule cells.; Authors - Jia Li
human,mouse	WB,CoIP	Life Sci; Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.; Authors - Shunying Jin ; KD Validated
human	WB	BMC Genomics; Paired guide RNA CRISPR-Cas9 screening for protein-coding genes and lncRNAs involved in transdifferentiation of human B-cells to macrophages.; Authors - Carme Arnan
mouse	WB	Adv Sci (Weinh); Fine-Tuning of Cholesterol Homeostasis Controls Erythroid Differentiation.; Authors - Zhiyuan Lu ; KD Validated