NFE2 Monoclonal antibody, PBS Only

• 

  WB analysis of HL-60 using 66436-1-Ig (same clone as 66436-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66436-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66436-1-Ig (same clone as 66436-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66436-1-PBS in a different storage buffer formulation.

NFE2 Monoclonal Antibody for WB, ELISA

Cat No. 66436-1-PBS

Clone No.1D7C8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

1D7C8, Leucine zipper protein NF E2, Leucine zipper protein NF-E2, NF E2, Nuclear factor, erythroid-derived 2 45 kDa subunit

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Product Information

66436-1-PBS targets NFE2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1570

  Product name: Recombinant human NFE2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-373 aa of BC005044

  Sequence: MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSIHPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQEDPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSGPVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEYALQQAADGTIFLVPRGTKMEATD

• Full Name: nuclear factor (erythroid-derived 2), 45kDa
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC005044
• Gene Symbol: NFE2
• Gene ID (NCBI): 4778
• RRID: AB_2881806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q16621
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.