Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17745-1-AP targets NFE2L3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12005 Product name: Recombinant human NFE2L3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 347-694 aa of BC068455 Sequence: SHTTNPEQTLPGTNLTGFLSPVDNHMRNLTSQDLLYDLDINIFDEINLMSLATEDNFDPIDVSQLFDEPDSDSGLSLDSSHNNTSVIKSNSSHSVCDEGAIGYCTDHESSSHHDLEGAVGGYYPEPSKLCHLDQSDSDFHGDLTFQHVFHNHTYHLQPTAPESTSEPFPWPGKSQKIRSRYLEDTDRNLSRDEQRAKALHIPFSVDEIVGMPVDSFNSMLSRYYLTDLQVSLIRDIRRRGKNKVAAQNCRKRKLDIILNLEDDVCNLQAKKETLKREQAQCNKAINIMKQKLHDLYHDIFSRLRDDQGRPVNPNHYALQCTHDGSILIVPKELVASGHKKETQKGKRK Predict reactive species |
| Full Name | nuclear factor (erythroid-derived 2)-like 3 |
| Calculated Molecular Weight | 694 aa, 76 kDa |
| Observed Molecular Weight | 76 kDa |
| GenBank Accession Number | BC068455 |
| Gene Symbol | NFE2L3 |
| Gene ID (NCBI) | 9603 |
| RRID | AB_2153211 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4A8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
The NFE2L3 Polyclonal antibody (Cat# 17745-1-AP) has 3 total citations. These appear in the journals Computational Methods and Programs in Biomedicine, Cancer Biology & Therapy, and Journal of Oncology. All citations involve Western blot in human samples and focus on oncology research, specifically esophageal squamous cell carcinoma, triple-negative breast cancer, and colon adenocarcinoma, examining NFE2L3's role in proliferation, radiotherapy resistance, and macrophage polarization.
| Species | Application | Title |
|---|---|---|
Comput Methods Programs Biomed Transcription factor NFE2L3 promotes the proliferation of esophageal squamous cell carcinoma cells and causes radiotherapy resistance by regulating IL-6
| ||
Cancer Biol Ther NRF3 suppresses the malignant progression of TNBC by promoting M1 polarization of macrophages via ROS/HMGB1 axis | ||
J Oncol miR-23b-3p Inhibits the Oncogenicity of Colon Adenocarcinoma by Directly Targeting NFE2L3. |
Reviews
What customers say
The single review for this antibody reports a 4-star rating. The user performed Western Blot on Beas2B cells at a 1:1000 dilution and confirmed that the antibody detected a band at the expected molecular weight of 130 kD. Overall, the feedback is positive, indicating the product performed as expected for its intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tao (Verified Customer) (02-21-2019) | This antibody could detect a band at molecular weight of 130 kD.
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