NFE2L3 Polyclonal antibody

• 

  WB analysis of human placenta using 17745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17745-1-AP (NFE2L3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 17745-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17745-1-AP (NFE2L3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NFE2L3 Polyclonal Antibody for WB, IF, ELISA

Cat No. 17745-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NF E2 related factor 3, NFE2 related factor 3, NFE2L3, NRF3

---

Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

17745-1-AP targets NFE2L3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12005

  Product name: Recombinant human NFE2L3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 347-694 aa of BC068455

  Sequence: SHTTNPEQTLPGTNLTGFLSPVDNHMRNLTSQDLLYDLDINIFDEINLMSLATEDNFDPIDVSQLFDEPDSDSGLSLDSSHNNTSVIKSNSSHSVCDEGAIGYCTDHESSSHHDLEGAVGGYYPEPSKLCHLDQSDSDFHGDLTFQHVFHNHTYHLQPTAPESTSEPFPWPGKSQKIRSRYLEDTDRNLSRDEQRAKALHIPFSVDEIVGMPVDSFNSMLSRYYLTDLQVSLIRDIRRRGKNKVAAQNCRKRKLDIILNLEDDVCNLQAKKETLKREQAQCNKAINIMKQKLHDLYHDIFSRLRDDQGRPVNPNHYALQCTHDGSILIVPKELVASGHKKETQKGKRK

• Full Name: nuclear factor (erythroid-derived 2)-like 3
• Calculated Molecular Weight: 694 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC068455
• Gene Symbol: NFE2L3
• Gene ID (NCBI): 9603
• RRID: AB_2153211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y4A8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Comput Methods Programs Biomed; Transcription factor NFE2L3 promotes the proliferation of esophageal squamous cell carcinoma cells and causes radiotherapy resistance by regulating IL-6; Authors - Tingting Chen ; KD Validated
human	WB	J Oncol; miR-23b-3p Inhibits the Oncogenicity of Colon Adenocarcinoma by Directly Targeting NFE2L3.; Authors - Guohong Huang
human	WB	Cancer Biol Ther; NRF3 suppresses the malignant progression of TNBC by promoting M1 polarization of macrophages via ROS/HMGB1 axis; Authors - Ping Xing

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tao (Verified Customer) (02-21-2019)

This antibody could detect a band at molecular weight of 130 kD.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Beas2B

NFE2L3 Antibody Western Blot, validation (1:1000 dilution) in Beas2B (Cat no:17745-1-AP)