NFIC Polyclonal antibody

• Home
• Products
• Primary Antibodies
• NFIC Polyclonal antibody (16399-1-AP)

NFIC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16399-1-AP Copied!

Publications (9)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

CCAAT-box-binding transcription factor, CTF, NF I, NF1-C, NF-I/C

• 

  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 16399-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 16399-1-AP

  IP result of anti-NFIC (IP:16399-1-AP, 4ug; Detection:16399-1-AP 1:1000) with HeLa cells lysate 3600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 16399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16399-1-AP (NFIC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 16399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16399-1-AP (NFIC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-3 using 16399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using NFIC antibody (16399-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-3 using 16399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using NFIC antibody (16399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for NFIC in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: PC-3 cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16399-1-AP targets NFIC in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9494

  Product name: Recombinant human NFIC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC012120

  Sequence: MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSSRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQ

• Full Name: nuclear factor I/C (CCAAT-binding transcription factor)
• Calculated Molecular Weight: 428aa,48 kDa; 508aa,57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC012120
• Gene Symbol: NFIC
• Gene ID (NCBI): 4782
• RRID: AB_2878252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08651
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Publications

What published studies show

NFIC antibody (Cat# 16399-1-AP) has 9 total citations published in journals including Cell Reports, International Journal of Oral Science, Cancer Cell International, Journal of Agricultural and Food Chemistry, Journal of Dental Research, Journal of Bone and Mineral Research, Molecular Carcinogenesis, Human Cell, and Archives of Oral Biology. Research fields span tooth development, chromatin remodeling, lung cancer, myoblast proliferation, m6A mRNA methylation, nasopharyngeal carcinoma, and oral squamous cell carcinoma.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IP,IHC	Int J Oral Sci; USP34 regulates tooth root morphogenesis by stabilizing NFIC.; Authors - Shuang Jiang
	WB,CoIP,ChIP	Cell Rep; Glucose-activated JMJD1A drives visceral adipogenesis via α-ketoglutarate-dependent chromatin remodeling; Authors - Chenxu Yang
human	WB,IP,IHC	Cancer Cell Int; NFIC mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to represses malignant phenotype of non-small cell lung cancer cells; Authors - Kesong Shi
bovine	WB,IF	J Agric Food Chem; Knockdown of NFIC Promotes Bovine Myoblast Proliferation through the CENPF/CDK1 Axis; Authors - Jianfang Wang ; KD Validated
mouse	IHC	J Dent Res; Essential Role of IFT140 in Promoting Dentinogenesis.; Authors - G Li
human	WB,IHC	J Bone Miner Res; METTL3-mediated m6 A mRNA methylation modulates tooth root formation via affecting NFIC translation.; Authors - Rui Sheng