NFIC Polyclonal antibody

NFIC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16399-1-AP

Synonyms

CTF, CTF5, NF I, NF I/C, NF1 C, NFI, NFI C, NFIC, Nuclear factor 1 C type, Nuclear factor 1/C, Nuclear factor I/C, TGGCA binding protein

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Validation Data Gallery

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  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16399-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16399-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16399-1-AP (NFIC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16399-1-AP

  IP result of anti-NFIC (IP:16399-1-AP, 4ug; Detection:16399-1-AP 1:1000) with HeLa cells lysate 3600ug.

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  IHC staining of human liver cancer using 16399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16399-1-AP (NFIC antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16399-1-AP (NFIC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 16399-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 16399-1-AP (NFIC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

16399-1-AP targets NFIC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFIC fusion protein Ag9494
• Full Name: nuclear factor I/C (CCAAT-binding transcription factor)
• Calculated Molecular Weight: 428aa,48 kDa; 508aa,57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC012120
• Gene Symbol: NFIC
• Gene ID (NCBI): 4782
• RRID: AB_2878252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Publications

Species	Application	Title
mouse	IHC	J Dent Res; Essential Role of IFT140 in Promoting Dentinogenesis.; Authors - G Li
human	WB,IHC	J Bone Miner Res; METTL3-mediated m6 A mRNA methylation modulates tooth root formation via affecting NFIC translation.; Authors - Rui Sheng
human,mouse	WB,IHC,IP	Int J Oral Sci; USP34 regulates tooth root morphogenesis by stabilizing NFIC.; Authors - Shuang Jiang
bovine	WB,IF	J Agric Food Chem; Knockdown of NFIC Promotes Bovine Myoblast Proliferation through the CENPF/CDK1 Axis; Authors - Jianfang Wang ; KD Validated
human	WB,IP,IHC	Cancer Cell Int; NFIC mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to represses malignant phenotype of non-small cell lung cancer cells; Authors - Kesong Shi
human	WB	Mol Carcinog; Neuroplastin-β mediates S100A8/A9-induced lung cancer disseminative progression.; Authors - I Wayan Sumardika