NFIL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

NFIL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 11773-1-AP

Synonyms

NFIL3A, Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4

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Validation Data Gallery

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  WB analysis using 11773-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11773-1-AP

  IP result of anti-NFIL3 (IP:11773-1-AP, 4ug; Detection:11773-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of mouse stomach using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11773-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFIL3 antibody (11773-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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  FC experiment of NK92 using 11773-1-AP

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (11773-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HepG2 cells, K562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse stomach tissue, human skin cancer tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: NK92, NK92 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Product Information

11773-1-AP targets NFIL3 in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFIL3 fusion protein Ag2380
• Full Name: nuclear factor, interleukin 3 regulated
• Calculated Molecular Weight: 462 aa, 51 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC008197
• Gene Symbol: NFIL3
• Gene ID (NCBI): 4783
• RRID: AB_2267287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFIL3, also named as E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227). The calculated molecular weight of NFIL3 is 51 kDa, but the modified NFIL3 is about 55-60 kDa.

Publications

Species	Application	Title
mouse	IF	Brain; Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.; Authors - Gen Shiihashi
mouse		Theranostics; The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.; Authors - Fangjun Yu
rat	WB	Neuropharmacology; Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area.; Authors - Cai Li
mouse	WB	Biochem Pharmacol; Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice; Authors - Min Chen
human	WB, ChIP	Front Med (Lausanne); NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation.; Authors - Honghu Tang
mouse	WB,chIP	Drug Metab Dispos; The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.; Authors - Min Chen ; KD Validated