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NFKB2 Polyclonal antibody

NFKB2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10409-2-AP

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NF kappa B, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100



Tested Applications

Positive WB detected inmulti-cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10409-2-AP targets NFKB2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFKB2 fusion protein Ag0662
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Calculated molecular weight 97 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC002844
Gene symbol NFKB2
Gene ID (NCBI) 4791
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody recognizes both p52 and p100.

Protocols

Product Specific Protocols
WB protocol for NFKB2 antibody 10409-2-APDownload protocol
IHC protocol for NFKB2 antibody 10409-2-APDownload protocol
IF protocol for NFKB2 antibody 10409-2-APDownload protocol
IP protocol for NFKB2 antibody 10409-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

A proteomics landscape of circadian clock in mouse liver.

Authors - Yunzhi Wang
humanWB

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff
mouseWB

J Hazard Mater

TiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2.

Authors - Shuang Li
humanWB

PLoS Pathog

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Authors - Muyu Xu
humanWB

Cell Death Dis

Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.

Authors - Xinhua Xiao
humanWB

Mol Ther Nucleic Acids

circPUM1 Promotes Tumorigenesis and Progression of Ovarian Cancer by Sponging miR-615-5p and miR-6753-5p.

Authors - Xue Guan