IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18220-1-AP

Synonyms

IKBA, IκB α, NFKBIA, Ikβ-α, IkB-alpha



Tested Applications

Positive WB detected inHL-60 cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue, human prostate cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 10 publications below

Product Information

18220-1-AP targets IkB Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IkB Alpha fusion protein Ag12979
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC004983
Gene Symbol IkB Alpha
Gene ID (NCBI) 4792
RRIDAB_2298441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Protocols

Product Specific Protocols
WB protocol for IkB Alpha antibody 18220-1-APDownload protocol
IHC protocol for IkB Alpha antibody 18220-1-APDownload protocol
IF protocol for IkB Alpha antibody 18220-1-APDownload protocol
IP protocol for IkB Alpha antibody 18220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

Activation of the NF-¿B pathway as a mechanism of alcohol enhanced progression and metastasis of human hepatocellular carcinoma.

Authors - Fei Wang
ratWB

Front Mol Neurosci

Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats.

Authors - Jin Jiang
ratWB

J Neuroinflammation

Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats.

Authors - Jian Zhan
mouseWB

Biomed Pharmacother

Tubeimoside I improves survival of mice in sepsis by inhibiting inducible nitric oxide synthase expression.

Authors - Minghao Luo
humanWB

Cancer Biol Ther

MiR-376a-3p increases cell apoptosis in acute myeloid leukemia by targeting MT1X.

Authors - Xiangke Xin
mouse,ratWB

Eur J Pharmacol

Tubeimoside I protects against sepsis-induced cardiac dysfunction via SIRT3.

Authors - Zhe Cheng