IkB Beta Polyclonal antibody

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  WB analysis using 12660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12660-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 12660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 12660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 12660-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12660-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 12660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human endometrial cancer using 12660-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 12660-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12660-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Beta antibody (12660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IkB Beta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12660-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IκB beta, IκB β, NFKBIB, IkB-B, IKBB

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: human colon tissue, human endometrial cancer tissue, human colon cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12660-1-AP targets IkB Beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IkB Beta fusion protein Ag3340
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
• Calculated Molecular Weight: 356 aa, 38 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC015528
• Gene Symbol: IkB Beta
• Gene ID (NCBI): 4793
• RRID: AB_2151611
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15653
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Toxicol Appl Pharmacol; Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.; Authors - Azubuike Peter Ebokaiwe
mouse	WB	Gut Microbes; Gut microbiota regulates host melatonin production through epithelial cell MyD88; Authors - Bingnan Liu