IkB Beta Polyclonal antibody

IkB Beta Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12660-1-AP

Synonyms

I kappa B beta, IkappaBbeta, IkB B, IkB beta, IKBB, NF kappa B inhibitor beta, NF kappa BIB, NFKBIB, TR interacting protein 9, TRIP 9, TRIP9



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human endometrial cancer tissue, human colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12660-1-AP targets IkB Beta in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IkB Beta fusion protein Ag3340
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Calculated Molecular Weight 356 aa, 38 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC015528
Gene Symbol NFKBIB
Gene ID (NCBI) 4793
RRIDAB_2151611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IkB Beta antibody 12660-1-APDownload protocol
IHC protocol for IkB Beta antibody 12660-1-APDownload protocol
IF protocol for IkB Beta antibody 12660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Toxicol Appl Pharmacol

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Authors - Azubuike Peter Ebokaiwe