IkB Beta Polyclonal antibody
IkB Beta Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12660-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells |
Positive IHC detected in | human colon tissue, human endometrial cancer tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
12660-1-AP targets IkB Beta in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IkB Beta fusion protein Ag3340 |
Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta |
Calculated molecular weight | 356 aa, 38 kDa |
Observed molecular weight | 46 kDa |
GenBank accession number | BC015528 |
Gene symbol | NFKBIB |
Gene ID (NCBI) | 4793 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for IkB Beta antibody 12660-1-AP | Download protocol |
IHC protocol for IkB Beta antibody 12660-1-AP | Download protocol |
IF protocol for IkB Beta antibody 12660-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Toxicol Appl Pharmacol Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells. |