|Positive WB detected in||A549 cells, HepG2 cells, HeLa cells, human heart tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human spleen tissue, human ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15370-1-AP targets NFS1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NFS1 fusion protein Ag3872|
|Full Name||NFS1 nitrogen fixation 1 homolog (S. cerevisiae)|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC018471|
|Gene ID (NCBI)||9054|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.
|Product Specific Protocols|
|WB protocol for NFS1 antibody 15370-1-AP||Download protocol|
|IHC protocol for NFS1 antibody 15370-1-AP||Download protocol|
|IF protocol for NFS1 antibody 15370-1-AP||Download protocol|
|IP protocol for NFS1 antibody 15370-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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