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  • KD/KO Validated

NFS1 Polyclonal antibody

NFS1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15370-1-AP

Synonyms

HUSSY 08, IscS, NFS1, NIFS



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, human heart tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15370-1-AP targets NFS1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFS1 fusion protein Ag3872
Full Name NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC018471
Gene symbol NFS1
Gene ID (NCBI) 9054
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for NFS1 antibody 15370-1-APDownload protocol
IHC protocol for NFS1 antibody 15370-1-APDownload protocol
IF protocol for NFS1 antibody 15370-1-APDownload protocol
IP protocol for NFS1 antibody 15370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Medchemcomm

4'-Phosphopantetheine and long acyl chain-dependent interactions are integral to human mitochondrial acyl carrier protein function.

Authors - Jaimeen D Majmudar
humanWB,IF

PLoS One

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

Authors - Ioana Ferecatu
  • KD Validated
mouseWB

FASEB J

PMID: 31530015

Authors - Rufeng Zhang
mouseWB

Cell Res

NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Authors - Tingting Hou