NFYB Polyclonal antibody

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• NFYB Polyclonal antibody (15868-1-AP)

NFYB Polyclonal Antibody for WB, ELISA

Cat No. 15868-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CAAT box DNA-binding protein subunit B, CBF A, CBF B, CBFA, CBFB

• 

  WB analysis using 15868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15868-1-AP (NFYB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15868-1-AP targets NFYB in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8495

  Product name: Recombinant human NFYB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-207 aa of BC005316

  Sequence: MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS

• Full Name: nuclear transcription factor Y, beta
• Calculated Molecular Weight: 207 aa, 23 kDa
• Observed Molecular Weight: ~30 kDa
• GenBank Accession Number: BC005316
• Gene Symbol: NFYB
• Gene ID (NCBI): 4801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25208
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFYB (Nuclear transcription factor Y subunit beta), also known as CBF-A/HAP3, is an essential subunit of the heterotrimeric NF-Y complex. NFYB has a histone-folding domain and binds NFYC in the cytoplasm; this dimer then enters the nucleus, recruits NFYA, and forms a trimer that specifically recognizes the CCAAT box, regulating genes involved in proliferation, apoptosis, and stress responses. (PMID: 37531316; PMID: 23977444)