Tested Applications
| Positive WB detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10129-2-AP targets NFYC in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0140 Product name: Recombinant human NFYC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-251 aa of BC005003 Sequence: MSTEGGFGGTSSSDAQQSLQSFWPRVMEEIRNLTVKDFRVQELPLARIKKIMKLDEDVKMISAEAPVLFAKAAQIFITELTLRAWIHTEDNKRRTLQRNDIAMAITKFDQFDFLIDIVPRDELKPPKRQEEVRQSVTPAEPVQYYFTLAQQPTAVQVQGQQQGQQTTSSTTTIQPGQIIIAQPQQGQTTPVTMQVGEGQQVQIVQAQPQGQAQQAQSGTGQTMQVMQQIITNTGEIQQIPVQLNAGQLQYI Predict reactive species |
| Full Name | nuclear transcription factor Y, gamma |
| Calculated Molecular Weight | 37 kDa, 50 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC005003 |
| Gene Symbol | NFYC |
| Gene ID (NCBI) | 4802 |
| RRID | AB_2152244 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13952 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NFYC, also named as Nuclear transcription factor Y subunit gamma or Transactivator HSM-1/2, is a 458 amino acid protein, which belongs to the NFYC/HAP5 subunit family. NFYC localizes in the nucleus and stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. SP1, NFYC and FOXO3, FOXO4 transcription factors are involved in the regulation of STK11 transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NFYC antibody 10129-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NFYC Polyclonal antibody (Cat# 10129-2-AP) has 1 citation. It was used in a Western Blot study published in Cell Research (2009), investigating pancreas-specific Pten deficiency, partial resistance to diabetes, and elevated hepatic AKT signaling in mouse models. The research field encompasses pancreatic biology, diabetes pathophysiology, and AKT signaling pathway regulation.
| Species | Application | Title |
|---|---|---|
Cell Res Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling. |
Reviews
What customers say
The single review for this product is highly positive (5/5). The reviewer used it for Western Blot on B cells at a 1:1000 dilution and reported excellent performance, noting a clear single band was detected in cell lysates. Overall, the feedback indicates reliable specificity and strong signal in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Annabel (Verified Customer) (02-12-2026) | Worked great, clear single band detected on cell lysates
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