NGB Polyclonal antibody

NGB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13499-1-AP

Synonyms

neuroglobin, NGB



Tested Applications

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13499-1-AP targets NGB in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGB fusion protein Ag4325
Full Name neuroglobin
Calculated molecular weight 151 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC032509
Gene symbol NGB
Gene ID (NCBI) 58157
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NGB antibody 13499-1-APDownload protocol
IHC protocol for NGB antibody 13499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Anat Rec (Hoboken)

Neuroglobin expression and function in the temporal cortex of bilirubin encephalopathy rats.

Authors - Jia-Heng Hu
WB

Life Sci

Hemin treatment protects neonatal rats from sevoflurane-induced neurotoxicity via the phosphoinositide 3-kinase/Akt pathway.

Authors - Fan Yang
WB

Front Neurosci

PMID: 31191229

Authors - Fan Yang
WB,IHC,IF

Cell Death Dis

Neuroglobin mediates neuroprotection of hypoxic postconditioning against transient global cerebral ischemia in rats through preserving the activity of Na+/K+ ATPases.

Authors - Haixia Wen