Tested Applications
| Positive WB detected in | fetal human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13499-1-AP targets NGB in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4325 Product name: Recombinant human NGB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC032509 Sequence: MERPEPELIRQSWRAVSRSPLEHGTVLFARLFALEPDLLPLFQYNCRQFSSPEDCLSSPEFLDHIRKVMLVIDAAVTNVEDLSSLEEYLASLGRKHRAVGVKLSSFSTVGESLLYMLEKCLGPAFTPATRAAWSQLYGAVVQAMSRGWDGE Predict reactive species |
| Full Name | neuroglobin |
| Calculated Molecular Weight | 151 aa, 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC032509 |
| Gene Symbol | NGB |
| Gene ID (NCBI) | 58157 |
| RRID | AB_2115391 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPG2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuroglobin (NGB), a monomeric 151-amino-acid protein, is a nerve globin family member widely present in vertebrates and is largely expressed in neurons of the central and peripheral nervous systems and in endocrine tissue (PMID: 33907006). NGB may increase oxygen availability and protect the central and peripheral nervous systems under hypoxic/ischemic conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NGB antibody 13499-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NGB Polyclonal antibody (Cat# 13499-1-AP) has 18 citations published in journals including Cell Death & Disease, Journal of Translational Medicine, Diabetes, Antioxidants, Life Sciences, Cells, Neuroscience, FEBS Letters, and Scientific Reports. Research fields span neuroprotection, cerebral ischemia/reperfusion, glioblastoma, diabetic nephropathy, atherosclerosis, neurotoxicity, ferroptosis, glaucoma, oxidative stress, autophagy, and cholesterol metabolism, with applications in WB, IHC, IF, and IP.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Neuroglobin mediates neuroprotection of hypoxic postconditioning against transient global cerebral ischemia in rats through preserving the activity of Na+/K+ ATPases. | ||
J Transl Med Neuroglobin-promoted autophagy inhibits the proliferation, migration and invasion of human glioblastoma cells through the EGFR/PI3K/AKT/mTOR signaling pathway.
| ||
Diabetes Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq. | ||
Antioxidants (Basel) Prunus cerasoides Extract and Its Component Compounds Upregulate Neuronal Neuroglobin Levels, Mediate Antioxidant Effects, and Ameliorate Functional Losses in the Mouse Model of Cerebral Ischemia. | ||
Lipids Health Dis Investigating the role of neuroglobin in cholesterol metabolism: a spotlight on brain-derived cells. | ||
CNS Neurosci Ther PTP1B Modulates Carotid Plaque Vulnerability in Atherosclerosis Through Rab5-PDGFRβ-Mediated Endocytosis Disruption and Apoptosis |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody at a 1:1000 dilution on liver tissue for Western Blot and reported that it works well for this application. Overall, the feedback indicates reliable performance for WB on liver samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-07-2025) | WORKS GOOD FOR WESTERN BLOT
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