p75NTR Polyclonal antibody
p75NTR Polyclonal Antibody for FC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, FC,ELISA
Conjugate
Unconjugated
Cat no : 55014-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human spleen tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
Product Information
55014-1-AP targets p75NTR in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | nerve growth factor receptor (TNFR superfamily, member 16) |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | NM_002507 |
| Gene symbol | NGFR |
| Gene ID (NCBI) | 4804 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p75NTR antibody 55014-1-AP | Download protocol |
| IHC protocol for p75NTR antibody 55014-1-AP | Download protocol |
| IP protocol for p75NTR antibody 55014-1-AP | Download protocol |
| FC protocol for p75NTR antibody 55014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ther Hypothermia Temp Manag Chipmunk Brain Is Resistant to Injury from Deep Hypothermic Circulatory Arrest During Cardiopulmonary Bypass. | ||
Psychopharmacology (Berl) PGC-1α-FNDC5-BDNF signaling pathway in skeletal muscle confers resilience to stress in mice subjected to chronic social defeat. | ||
Exp Ther Med Involvement of neurotrophic signaling in doxorubicin-induced cardiotoxicity. | ||
Med Sci Monit Feasibility of Diffusion Tensor Imaging for Assessing Functional Recovery in Rats with Olfactory Ensheathing Cell Transplantation After Contusive Spinal Cord Injury (SCI). | ||
Int J Mol Sci Effects of different sera conditions on olfactory ensheathing cells in vitro. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Renato (Verified Customer) (02-27-2019) | Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.
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