p75NTR Polyclonal antibody
p75NTR Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, FC, ELISA
Cat no : 55014-1-AP
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue, human spleen tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 10 publications below|
|IHC||See 3 publications below|
|IF||See 7 publications below|
55014-1-AP targets p75NTR in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||nerve growth factor receptor (TNFR superfamily, member 16)|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||70-75 kDa|
|GenBank accession number||NM_002507|
|Gene ID (NCBI)||4804|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.
|Product Specific Protocols|
|WB protocol for p75NTR antibody 55014-1-AP||Download protocol|
|IHC protocol for p75NTR antibody 55014-1-AP||Download protocol|
|IP protocol for p75NTR antibody 55014-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Exp Med
CXCR4 regulates Plasmodium development in mouse and human hepatocytes.
LMO1 Plays an Oncogenic Role in Human Glioma Associated With NF-kB Pathway.
Stem Cell Res Ther
NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.
J Mol Med (Berl)
MESP2 variants contribute to conotruncal heart defects by inhibiting cardiac neural crest cell proliferation.
Stem Cells Dev
Exosomes from Hair Follicle Epidermal Neural Crest Stem Cells Promote Acellular Nerve Allografts to Bridge Rat Facial Nerve Defects
Int J Mol Sci
Astaxanthin Ameliorates Ischemic-Hypoxic-Induced Neurotrophin Receptor p75 Upregulation in the Endothelial Cells of Neonatal Mouse Brains.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Renato (Verified Customer) (02-27-2019)
Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.