p75NTR Polyclonal antibody

p75NTR Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 55014-1-AP

Synonyms

CD271, Gp80 LNGFR, NGF receptor, NGFR, p75, p75 ICD, p75(NTR), p75NTR, TNFRSF16

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Validation Data Gallery

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  WB analysis of mouse brain using 55014-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55014-1-AP (p75NTR antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55014-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55014-1-AP (p75NTR antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55014-1-AP

  IP Result of anti-p75NTR (IP:55014-1-AP, 3ug; Detection:55014-1-AP 1:300) with mouse brain tissue lysate 5000ug.

• 

  IHC staining of mouse brain using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55014-1-AP (p75NTR antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55014-1-AP (p75NTR antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 55014-1-AP (p75NTR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 55014-1-AP (p75NTR antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of SH-SY5Y using 55014-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug p75NTR antibody (55014-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human spleen tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Product Information

55014-1-AP targets p75NTR in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nerve growth factor receptor (TNFR superfamily, member 16)
• Calculated molecular weight: 45 kDa
• Observed molecular weight: 70-75 kDa
• GenBank accession number: NM_002507
• Gene symbol: NGFR
• Gene ID (NCBI): 4804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.

Publications

Species	Application	Title
rat	WB	Ther Hypothermia Temp Manag; Chipmunk Brain Is Resistant to Injury from Deep Hypothermic Circulatory Arrest During Cardiopulmonary Bypass.; Authors - Xuan Jiang
mouse	WB	Psychopharmacology (Berl); PGC-1α-FNDC5-BDNF signaling pathway in skeletal muscle confers resilience to stress in mice subjected to chronic social defeat.; Authors - Gaofeng Zhan
human	WB	Connect Tissue Res; Relevance of meniscal cell regional phenotype to tissue engineering.; Authors - Shawn P Grogan
rat	WB	Exp Ther Med; Involvement of neurotrophic signaling in doxorubicin-induced cardiotoxicity.; Authors - Dehua Liao
rat	IF	Med Sci Monit; Feasibility of Diffusion Tensor Imaging for Assessing Functional Recovery in Rats with Olfactory Ensheathing Cell Transplantation After Contusive Spinal Cord Injury (SCI).; Authors - Mengchao Gu
human,mouse	IHC	J Toxicol Sci; Lidocaine prevents breast cancer growth by targeting neuronatin to inhibit nerve fibers formation.; Authors - Bingda Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Renato (Verified Customer) (02-27-2019)

Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue

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