p75NTR Polyclonal antibody

p75NTR Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 55014-1-AP

Synonyms

CD271, Gp80 LNGFR, NGF receptor, NGFR, p75, p75 ICD, p75(NTR), p75NTR, TNFRSF16

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Validation Data Gallery

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  WB analysis of mouse brain using 55014-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55014-1-AP (p75NTR antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55014-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55014-1-AP (p75NTR antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55014-1-AP

  IP Result of anti-p75NTR (IP:55014-1-AP, 3ug; Detection:55014-1-AP 1:300) with mouse brain tissue lysate 5000ug.

• 

  IHC staining of mouse brain using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55014-1-AP (p75NTR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 55014-1-AP (p75NTR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 55014-1-AP (p75NTR antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55014-1-AP (p75NTR antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 55014-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55014-1-AP (p75NTR antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of SH-SY5Y using 55014-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug p75NTR antibody (55014-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human spleen tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 12 publications below
• IHC: See 5 publications below

Product Information

55014-1-AP targets p75NTR in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nerve growth factor receptor (TNFR superfamily, member 16)
• Calculated molecular weight: 45 kDa
• Observed molecular weight: 70-75 kDa
• GenBank accession number: NM_002507
• Gene symbol: NGFR
• Gene ID (NCBI): 4804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.

Publications

Species	Application	Title
mouse		J Exp Med; CXCR4 regulates Plasmodium development in mouse and human hepatocytes.; Authors - Hironori Bando
mouse	WB	Int J Mol Sci; Direct Enhancement Effect of Hippocampal Cholinergic Neurostimulating Peptide on Cholinergic Activity in the Hippocampus; Authors - Yuta Madokoro
human	IHC	Int J Mol Sci; Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review; Authors - Hiroshi Toshida
human,mouse	WB,IHC	Front Oncol; LMO1 Plays an Oncogenic Role in Human Glioma Associated With NF-kB Pathway.; Authors - Lei Gao
human	WB	Stem Cell Res Ther; NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.; Authors - Ke Wu
human		J Mol Med (Berl); MESP2 variants contribute to conotruncal heart defects by inhibiting cardiac neural crest cell proliferation.; Authors - Erge Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Renato (Verified Customer) (02-27-2019)

Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue

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