p75NTR Polyclonal antibody

p75NTR Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 55014-1-AP

Synonyms

CD271, Gp80 LNGFR, NGF receptor, NGFR, p75, p75 ICD, p75(NTR), p75NTR, TNFRSF16



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human spleen tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55014-1-AP targets p75NTR in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nerve growth factor receptor (TNFR superfamily, member 16)
Calculated molecular weight 45 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberNM_002507
Gene symbol NGFR
Gene ID (NCBI) 4804
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.

Protocols

Product Specific Protocols
WB protocol for p75NTR antibody 55014-1-APDownload protocol
IHC protocol for p75NTR antibody 55014-1-APDownload protocol
IP protocol for p75NTR antibody 55014-1-APDownload protocol
FC protocol for p75NTR antibody 55014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Ther Hypothermia Temp Manag

Chipmunk Brain Is Resistant to Injury from Deep Hypothermic Circulatory Arrest During Cardiopulmonary Bypass.

Authors - Xuan Jiang
mouseWB

Psychopharmacology (Berl)

PGC-1α-FNDC5-BDNF signaling pathway in skeletal muscle confers resilience to stress in mice subjected to chronic social defeat.

Authors - Gaofeng Zhan
humanWB

Connect Tissue Res

Relevance of meniscal cell regional phenotype to tissue engineering.

Authors - Shawn P Grogan
ratWB

Exp Ther Med

Involvement of neurotrophic signaling in doxorubicin-induced cardiotoxicity.

Authors - Dehua Liao
ratIF

Med Sci Monit

Feasibility of Diffusion Tensor Imaging for Assessing Functional Recovery in Rats with Olfactory Ensheathing Cell Transplantation After Contusive Spinal Cord Injury (SCI).

Authors - Mengchao Gu
ratIF

Int J Mol Sci

Effects of different sera conditions on olfactory ensheathing cells in vitro.

Authors - Meng Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Renato (Verified Customer) (02-27-2019)

Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissue