NHE1 Recombinant monoclonal antibody, PBS Only

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• NHE1 Recombinant monoclonal antibody, PBS Only (87385-1-PBS)

NHE1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87385-1-PBS

Clone No.252542A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

SLC9A1, APNH, APNH1, FLJ42224, Na(+)/H(+) antiporter, amiloride-sensitive

• 

  WB analysis using 87385-1-RR (same clone as 87385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87385-1-RR (NHE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87385-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

87385-1-PBS targets NHE1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 1
• Calculated Molecular Weight: 815 aa, 91 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC012121
• Gene Symbol: NHE1
• Gene ID (NCBI): 6548
• RRID: AB_3745504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19634
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).