NHE2 Polyclonal antibody

NHE2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25105-1-AP

Synonyms

Na(+)/H(+) exchanger 2, NHE 2, NHE2, NHE2/SLC9A2, SLC9A2, Sodium/hydrogen exchanger 2

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Validation Data Gallery

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  WB analysis using 25105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25105-1-AP (NHE2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat brain tissue, mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25105-1-AP targets NHE2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NHE2 fusion protein Ag18801
• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 2
• Calculated Molecular Weight: 812 aa, 92 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC136377
• Gene Symbol: SLC9A2
• Gene ID (NCBI): 6549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHE2, also known as SLC9A2, is a protein-coding gene mainly at the top of gastrointestinal epithelial cells. It mainly encodes a member of the sodium-hydrogen exchanger (NHE) protein family, which is localized to the apical membrane and is easily degraded by lysosomes, resulting in a short half-life. NHE2 is involved in sodium ion transport by exchanging intracellular hydrogen ions into external sodium ions, and helps in the regulation of cell pH and volume to eliminate acids produced by active metabolism or against adverse environmental conditions. NHE2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands (PMID: 37688782). The observed molecular weight of NHE2 is 70 kDa, which might be the unmodified form of NHE2 (PMID: 10444453, 10666043).