XLF Polyclonal antibody

XLF Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 11888-1-AP

Synonyms

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor



Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

11888-1-AP targets XLF in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XLF fusion protein Ag2496
Full Name nonhomologous end-joining factor 1
Calculated molecular weight 35 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC030986
Gene symbol NHEJ1
Gene ID (NCBI) 79840
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.

Protocols

Product Specific Protocols
WB protocol for XLF antibody 11888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FEBS Lett

RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway.

Authors - Yiran Zhong
humanWB

Int J Biochem Cell Biol

Targeting Ku86 enhances X-ray-induced radiotherapy sensitivity in serous ovarian cancer cells.

Authors - Qian Ma
humanWB

PLoS One

5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation.

Authors - Wei Jiang
humanWB

J Med Genet

Haploinsufficiency in non-homologous end joining factor 1 induces ovarian dysfunction in humans and mice.

Authors - Guoqing Li