|Positive WB detected in||COLO 320 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Sample-dependent, check data in validation data gallery|
11888-1-AP targets XLF in WB,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||XLF fusion protein Ag2496|
|Full Name||nonhomologous end-joining factor 1|
|Calculated molecular weight||35 kDa|
|Observed molecular weight||33-38 kDa|
|GenBank accession number||BC030986|
|Gene ID (NCBI)||79840|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.
RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway.
Int J Biochem Cell Biol
Targeting Ku86 enhances X-ray-induced radiotherapy sensitivity in serous ovarian cancer cells.
5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation.
J Med Genet
Haploinsufficiency in non-homologous end joining factor 1 induces ovarian dysfunction in humans and mice.