NHLRC2 Polyclonal antibody

NHLRC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29416-1-AP

Synonyms

DKFZp779F115, FLJ20147, FLJ25621, FLJ33312, NHL repeat containing 2, NHLRC2

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Validation Data Gallery

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  WB analysis using 29416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29416-1-AP (NHLRC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29416-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29416-1-AP (NHLRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29416-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29416-1-AP (NHLRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-251 cells, mouse spleen tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29416-1-AP targets NHLRC2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NHLRC2 fusion protein Ag30159
• Full Name: NHL repeat containing 2
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: NM_198514
• Gene Symbol: NHLRC2
• Gene ID (NCBI): 374354
• RRID: AB_2918302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.