Tested Applications
| Positive WB detected in | SH-SY5Y cells, Caco-2 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15128-1-AP targets NHP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7251 Product name: Recombinant human NHP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-153 aa of BC000009 Sequence: MTKIKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMVLAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVKPHEEYQEAYDECLEEVQSLPLPL Predict reactive species |
| Full Name | NHP2 ribonucleoprotein homolog (yeast) |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17-20 kDa |
| GenBank Accession Number | BC000009 |
| Gene Symbol | NHP2 |
| Gene ID (NCBI) | 55651 |
| RRID | AB_2267290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NX24 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IHC protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IP protocol for NHP2 antibody 15128-1-AP | Download protocol |
| WB protocol for NHP2 antibody 15128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NHP2 Polyclonal antibody (Cat# 15128-1-AP) has 21 citations across journals including Nature, Science, Cell, Nucleic Acids Research, EMBO Journal, Cell Reports, EMBO Reports, Genome Biology, Journal of Translational Medicine, Human Molecular Genetics, Pathology, Biomedicines, Experimental Eye Research, Histochemistry and Cell Biology, Molecular Cell Biology, RNA, and bioRxiv. Research fields span telomerase biology, H/ACA RNP/snoRNA function, ribosome biogenesis, DNA damage response, RNA pseudouridylation, and cancer (gastric, colorectal, prostate, uveal melanoma).
| Species | Application | Title |
|---|---|---|
Science Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerization | ||
Cell snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification | ||
Nucleic Acids Res N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation. | ||
