Entactin Polyclonal antibody

Entactin Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 13766-1-AP

Synonyms

Entactin, NID, NID 1, NID1, Nidogen 1



Tested Applications

Positive WB detected inhuman placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IP detected inhuman placenta tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13766-1-AP targets Entactin in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Entactin fusion protein Ag4749
Full Name nidogen 1
Calculated molecular weight 1247 aa, 136 kDa
Observed molecular weight 136-150 kDa
GenBank accession numberBC045606
Gene symbol NID1
Gene ID (NCBI) 4811
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Entactin antibody 13766-1-APDownload protocol
IF protocol for Entactin antibody 13766-1-APDownload protocol
IP protocol for Entactin antibody 13766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols