Entactin Polyclonal antibody

Entactin Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13766-1-AP

Synonyms

Entactin, NID, NID 1, NID1, Nidogen 1



Tested Applications

Positive WB detected inhuman placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IP detected inhuman placenta tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13766-1-AP targets Entactin in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Entactin fusion protein Ag4749
Full Name nidogen 1
Calculated molecular weight 1247 aa, 136 kDa
Observed molecular weight 136-150 kDa
GenBank accession numberBC045606
Gene symbol NID1
Gene ID (NCBI) 4811
RRIDAB_10642564
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Entactin antibody 13766-1-APDownload protocol
IF protocol for Entactin antibody 13766-1-APDownload protocol
IP protocol for Entactin antibody 13766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,CoIP

Bioact Mater

Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion.

Authors - Pengzhen Cheng
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni