NIF3L1 Polyclonal antibody

NIF3L1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10549-1-AP

Synonyms

ALS2CR1, CALS 7, MDS015, My018, NIF3 like protein 1, NIF3L1

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Validation Data Gallery

• 

  WB analysis using 10549-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10549-1-AP (NIF3L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10549-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10549-1-AP (NIF3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10549-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10549-1-AP (NIF3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10549-1-AP (NIF3L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, PC-3 cells, PC-12 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10549-1-AP targets NIF3L1 in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NIF3L1 fusion protein Ag0819
• Full Name: NIF3 NGG1 interacting factor 3-like 1 (S. pombe)
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa, 39 kDa
• GenBank Accession Number: BC007654
• Gene Symbol: NIF3L1
• Gene ID (NCBI): 60491
• RRID: AB_2153439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.