Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11789-1-AP targets NIPSNAP3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2377 Product name: Recombinant human NIPSNAP3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-247 aa of BC017914 Sequence: MLVLRSGLTKALASRTLAPQVCSSFATGPRQYDGTFYEFRTYYLKPSNMNAFMENLKKNIHLRTSYSELVGFWSVEFGGRTNKVFHIWKYDNFAHRAEVRKALANCKEWQEQSIIPNLARIDKQETEITYLIPWSKLEKPPKEGVYELAVFQMKPGGPALWGDAFERAINAHVNLGYTKVVGVFHTEYGELNRVHVLWWNESADSRAAGRHKSHEDPRVVAAVRESVNYLVSQQNMLLIPASFSPLK Predict reactive species |
| Full Name | nipsnap homolog 3B (C. elegans) |
| Calculated Molecular Weight | 247 aa, 28 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC017914 |
| Gene Symbol | NIPSNAP3B |
| Gene ID (NCBI) | 55335 |
| RRID | AB_2151306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BS92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NIPSNAP3B is a member of the NIPSNAP family with putative roles in vesicular trafficking (PMID: 15177564). The gene of NIPSNAP3B maps to chromosome 9q31.3, and encodes a 247-amino-acid protein with a calculated molecular mass of 28 kDa. NIPSNAP3B gene has been reported to be highly associated with low bone mineral density (BMD) variation in postmenopausal Caucasian women (PMID: 19922823).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NIPSNAP3B antibody 11789-1-AP | Download protocol |
| WB protocol for NIPSNAP3B antibody 11789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NIPSNAP3B Polyclonal antibody (catalog number 11789-1-AP) has a total of 1 citation. It appears in Scientific Reports (2024), used for Western blot in human samples. The associated research field is mitochondrial quality control, specifically how clarithromycin-binding proteins NIPSNAP1 and 2 regulate cytokine production.
| Species | Application | Title |
|---|---|---|
Sci Rep The clarithromycin-binding proteins NIPSNAP1 and 2 regulate cytokine production through mitochondrial quality control |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application using LUHMES cells at a 1:1000 dilution. The reviewer, a PhD student studying Parkinson's disease pathophysiology, tested the antibody alongside a NIPSNAP3B antibody and reported that it produced a very precise result with one clear band, indicating strong specificity and reliable performance for their research.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kristina (Verified Customer) (01-21-2022) | Beside NIPSNAP3A, I also tested an antibody for NIPSNAP3B as I study these proteins and their relevance in pathophysiology of Parkinson's disease for my PhD thesis. Also the NIPSNAP3B antibody showed a very precise result with one clear band.
|
