NISCH Polyclonal antibody

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• NISCH Polyclonal antibody (13813-1-AP)

NISCH Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13813-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

I1R, I-1 receptor candidate protein, I-1, I 1 receptor candidate protein, I 1

• 

  WB analysis using 13813-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13813-1-AP (NISCH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 13813-1-AP

  IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with Jurkat cells lysate 1560 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 13813-1-AP

  IP result of anti-NISCH (IP:13813-1-AP, 4ug; Detection:13813-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 13813-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13813-1-AP (NISCH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: Jurkat cells, mouse brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13813-1-AP targets NISCH in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4880

  Product name: Recombinant human NISCH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1155-1504 aa of BC038102

  Sequence: NEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPVELTG

• Full Name: nischarin
• Calculated Molecular Weight: 1504 aa, 167 kDa
• Observed Molecular Weight: 190-200 kDa
• GenBank Accession Number: BC038102
• Gene Symbol: NISCH
• Gene ID (NCBI): 11188
• RRID: AB_10693526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2I1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Anti-Nischarin (Cat# 13813-1-AP) has 4 citations published in Phytomedicine, International Immunopharmacology, Anesthesia & Analgesia, and Biochemical and Biophysical Research Communications. Research fields include breast cancer (NOX4 modulation, Wnt3α/β-catenin pathway, apoptosis, migration and invasion) and perioperative neurocognitive disorders following traumatic brain injury in mice.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Phytomedicine; Anti-tumor effects of Cinnamomum camphora essential oil in breast cancer via NOX4 modulation by (+)-2-bornanone; Authors - Ruzhen Zheng
human	WB	Int Immunopharmacol; Preliminary investigation on the mechanism of baicalein regulating the effects of Nischarin on invasion and apoptosis of human breast cancer cells MCF-7 through Wnt3α/β-catenin pathway; Authors - Gaojian He
mouse	IF	Anesth Analg; Dexmedetomidine Ameliorates Perioperative Neurocognitive Disorders by Suppressing Monocyte-Derived Macrophages in Mice With Preexisting Traumatic Brain Injury.; Authors - Natsumi Kii
human	WB,IF	Biochem Biophys Res Commun; Expression of Nischarin negatively correlates with estrogen receptor and alters apoptosis, migration and invasion in human breast cancer.; Authors - Chan Chang