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WB analysis of HeLa using 68169-1-Ig (same clone as 68169-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
WB analysis using 68169-1-Ig (same clone as 68169-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
WB analysis of pig liver using 68169-1-Ig (same clone as 68169-1-PBS)
pig liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 68169-1-Ig (same clone as 68169-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
WB analysis of rat liver using 68169-1-Ig (same clone as 68169-1-PBS)
rat liver tissue were subjected to SDS PAGE followed by western blot with 68169-1-Ig (NIT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 68169-1-Ig (same clone as 68169-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68169-1-Ig (NIT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.
IF Staining of U-251 using 68169-1-Ig (same clone as 68169-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NIT2 antibody (68169-1-Ig, Clone: 1E5B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68169-1-PBS in a different storage buffer formulation.

NIT2 Monoclonal antibody, PBS Only

NIT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68169-1-PBS

Clone No.1E5B1

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

NIT2, nitrilase family, member 2, Nitrilase homolog 2, Omega amidase NIT2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

68169-1-PBS targets NIT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity	Human, mouse, rat, pig
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag9889 Product name: Recombinant human NIT2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-276 aa of BC020620 Sequence: MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIPGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEALAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMKKP Predict reactive species
Full Name	nitrilase family, member 2
Calculated Molecular Weight	276 aa, 31 kDa
Observed Molecular Weight	31 kDa
GenBank Accession Number	BC020620
Gene Symbol	NIT2
Gene ID (NCBI)	56954
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NQR4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.