NKCC1/SLC12A2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86201-1-RR (same clone as 86201-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86201-1-RR (same clone as 86201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 86201-1-RR (same clone as 86201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 86201-1-RR (same clone as 86201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 86201-1-RR (same clone as 86201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86201-1-RR (NKCC1/SLC12A2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86201-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86201-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86201-1-RR against Human NKCC1/SLC12A2 were performed. The affinity constant is below 1 pM.

NKCC1/SLC12A2 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86201-1-PBS

Clone No.250829D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter, BSC

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Product Information

86201-1-PBS targets NKCC1/SLC12A2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4991

  Product name: Recombinant human SLC12A2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 759-1150 aa of BC033003

  Sequence: GSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKFYEPC

• Full Name: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC033003
• Gene Symbol: NKCC1/SLC12A2
• Gene ID (NCBI): 6558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55011
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).