|Positive WB detected in||HEK-293 cells, HepG2 cells|
|Positive IP detected in||HEK-293 cells,|
|Positive IF detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10174-1-AP targets NKIRAS2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||NKIRAS2 fusion protein Ag0221|
|Full Name||NFKB inhibitor interacting Ras-like 2|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC007450|
|Gene ID (NCBI)||28511|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andrea (Verified Customer) (09-18-2020)
The antibody works great in Western Blot. Good signal and specificity, which was tested on knockout cells.