NKIRAS2 Polyclonal antibody

NKIRAS2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 10174-1-AP

Synonyms

Kappa B Ras protein 2, kappaB Ras2, KBRAS2, NKIRAS2



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHEK-293 cells,
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10174-1-AP targets NKIRAS2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKIRAS2 fusion protein Ag0221
Full Name NFKB inhibitor interacting Ras-like 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC007450
Gene symbol NKIRAS2
Gene ID (NCBI) 28511
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.

Protocols

Product Specific Protocols
WB protocol for NKIRAS2 antibody 10174-1-APDownload protocol
IF protocol for NKIRAS2 antibody 10174-1-APDownload protocol
IP protocol for NKIRAS2 antibody 10174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

κB-Ras and Ral GTPases regulate acinar to ductal metaplasia during pancreatic adenocarcinoma development and pancreatitis.

Authors - Stephanie Beel

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Andrea (Verified Customer) (09-18-2020)

The antibody works great in Western Blot. Good signal and specificity, which was tested on knockout cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: pancreas, different human and mouse cell lines